Korean J Parasitol.  2017 Feb;55(1):15-20. 10.3347/kjp.2017.55.1.15.

Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis

Affiliations
  • 1Laboratory Animal Center of Nantong University, Nantong 226001, China. shaoyx@ntu.edu.cn
  • 2Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 21009, China.
  • 3Nantong Entry-Exit Inspection and Quarantine Bureau, Nantong 226004, China.

Abstract

The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

Keyword

Eimeria stiedae; rabbit; sporozoite; immunoproteomics; vaccine target

MeSH Terms

Coccidiosis*
Computational Biology
Eimeria
Electrophoresis
HSP70 Heat-Shock Proteins
Immunoblotting
Mass Spectrometry
Rabbits
Sporozoites
HSP70 Heat-Shock Proteins
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