Korean J Vet Res.  2011 Sep;51(3):249-252.

An animal model using Eimeria live vaccine and to study coccidiosis protozoa pathogenesis

Affiliations
  • 1Center for Animal Resources Development, Wonkwang University, Iksan 570-749, Korea. kimoj@wku.ac.kr
  • 2Institute of Animal Experiment & Efficacy Evaluation, Wonkwang University, Iksan 570-749, Korea.
  • 3Institute of Biotechnology, Wonkwang University, Iksan 570-749, Korea.

Abstract

Cell culture systems for the protozoan Eimeria are not yet available. The present study was conducted to develop an animal model system by inoculating animals with a live Eimeria vaccine. This study was conducted on 3-day-old chickens (n = 20) pretreated with cyclophosphamide. The chickens were divided into 2 groups: the control group (n = 10) and the inoculated group that received the live Eimeria vaccine (n = 10). During the study period, we compared the clinical signs, changes in body weight, and number of oocysts shed in the feces of the control and inoculated group. This study showed that oocyst shedding was significantly higher in the chickens inoculated with live Eimeria oocysts than in the control chickens. Moreover, body weight gain was lesser in the animals in the inoculated group than in the control animals. Fecal oocyst shedding was observed in the inoculated animals. On the basis of these findings, we suggest that live Eimeria vaccination with cyclophosphamide pretreatment may be used to obtain an effective animal model for studying protozoan infections. This animal study model may eliminate the need for a tedious continuous animal inoculation process every 6 months because the live coccidiosis vaccine contains live oocysts.

Keyword

animal model; coccidiosis; cyclophosphamide; Eimeria; Protozoa

MeSH Terms

Animals
Body Weight
Cell Culture Techniques
Chickens
Coccidiosis
Cyclophosphamide
Eimeria
Feces
Models, Animal
Oocysts
Protozoan Infections
Vaccination
Cyclophosphamide
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