Int Neurourol J.  2016 Jun;20(2):114-121. 10.5213/inj.1632578.289.

Anti-interleukin-33 Reduces Ovalbumin-Induced Nephrotoxicity and Expression of Kidney Injury Molecule-1

• Park GH
• Shinn HK
• Kang JH
• Na WJ
• Kim YH
• Park CS

• KMID: 2365742
• DOI: http://doi.org/10.5213/inj.1632578.289

Abstract

PURPOSE
To evaluate the effect of anti-interleukin-33 (anti-IL-33) on a mouse model of ovalbumin (OVA)-induced acute kidney injury (AKI).
METHODS
Twenty-four female BALB/c mice were assigned to 4 groups: group A (control, n=6) was administered sterile saline intraperitoneally (i.p.) and intranasally (i.n.); group B (allergic, n=6) was administered i.p./i.n. OVA challenge; group C (null treatment, n=6) was administered control IgG i.p. before OVA challenge; and group D (anti-IL-33, n=6) was pretreated with 3.6 µg of anti-IL-33 i.p. before every OVA challenge. The following were evaluated after sacrifice: serum blood urea nitrogen and creatinine levels, Kidney injury molecule-1 gene (Kim-1) and protein (KIM-1) expression in renal parenchyma, and expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), phosphorylated endothelial NOS (p-eNOS), and phosphorylated AMP kinase (p-AMPK) proteins in renal parenchyma.
RESULTS
After OVA injection and intranasal challenge, mice in groups B and C showed significant increases in the expression of Kim-1 at both the mRNA and protein levels. After anti-IL-33 treatment, mice in group D showed significant Kim-1 down-regulation at the mRNA and protein levels. Group D also showed significantly lower COX-2 protein expression, marginally lesser iNOS expression than groups B and C, and p-eNOS and p-AMPK expression at baseline levels.
CONCLUSIONS
Kim-1 could be a useful marker for detecting early-stage renal injury in mouse models of OVA-induced AKI. Further, anti-IL-33 might have beneficial effects on these mouse models.

Keyword

Acute Kidney Injury; Interleukin-33; Ovalbumin; Kidney Injury Molecule-1

MeSH Terms

Acute Kidney Injury
Adenylate Kinase
Animals
Blood Urea Nitrogen
Creatinine
Cyclooxygenase 2
Down-Regulation
Female
Humans
Immunoglobulin G
Interleukin-33
Kidney*
Mice
Nitric Oxide Synthase Type II
Ovalbumin
Ovum
RNA, Messenger
Adenylate Kinase
Creatinine
Cyclooxygenase 2
Immunoglobulin G
Interleukin-33
Nitric Oxide Synthase Type II
Ovalbumin
RNA, Messenger