J Korean Neurol Assoc.
1998 Aug;16(4):536-546.
Study of dopamine transporter in the rat 6-hydroxydopamine Parkinson's disease model
- Affiliations
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- 1Department of Neurology Hallym University College of Medicine.
- 2Department of Nuclear Medicine, Seoul National University College of Medicine.
- 3Department of Pharmacology, Seoul National University College of Medicine.
- 4Department of Neurology, Seoul National University College of Medicine.
- 5Department of Neurology Inha University College of Medicine.
Abstract
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BACKGROUND: Neuroprotective therapy is essential in the management of Parkinson's disease(PD). As symptomatic benefit of a treatment may clinically mask the disease progression, an evaluation of the effect of a neuroprotective therapy should be based on objective measurement of in vivo dopaminergic integrity: Nuclear imaging techniques such as SPECT or PET can visualize dopaminergic system using dopamine transporter ligands and show the promise for this purpose. The objective of this study is to examine the changes of dopamine transporter in the animal model of PD and those correlations with behavioral and biochemical changes.
METHODS
We injected 6-hydroxydopamine into the substantia nigra in Sprague-Dawley rats to establish the unilateral PD model, and examined the rotation response after apomorphine injection as a behavioral aspect of the animal model. And we also measured the dopamine and DOPAC level in the striata and the dopamine transporter by [3H]-mazindol autoradiography.
RESULTS
We observed that the rats showed turning behavior only after severe reduction of dopamine and DOPAC. There was a strong inverse correlation between rotation behavior and striatal dopamine, DOPAC and dopamine transporter density. There was a positive and strong linear-correlation between dopamine transporter density and dopamine or DOPAC levels.
CONCLUSION
Measurement of dopamine transporter gives a good estimate of striatal dopamine level in an animal model of PD. In vivo measurement of dopamine transporter will give an objective information on the integrity of presynaptic nigrostriatal dopaminergic system.
MeSH Terms
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3,4-Dihydroxyphenylacetic Acid
Animals
Apomorphine
Autoradiography
Disease Progression
Dopamine Plasma Membrane Transport Proteins*
Dopamine*
Ligands
Masks
Models, Animal
Oxidopamine*
Parkinson Disease*
Rats*
Rats, Sprague-Dawley
Substantia Nigra
Tomography, Emission-Computed, Single-Photon
3,4-Dihydroxyphenylacetic Acid
Apomorphine
Dopamine
Dopamine Plasma Membrane Transport Proteins
Ligands
Oxidopamine
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