J Korean Ophthalmol Soc.  2005 Nov;46(11):1903-1910.

Effect of Oxidative Stress on the Expression of Gelatinases in Cultured RPE

• Park YC
• Choi GJ

Abstract

PURPOSE
To evaluate the expression pattern of gelatinases in the human retinal pigment epithelial cell line ARPE-19 in response to oxidative stress. METHODS: ARPE-19 cells were exposed to paraquat for 72 hours. The cells were assayed for the mRNA expression of gelatinase A and gelatinase B by reverse transcriptase polymerase chain reaction (RT-PCR). The conditioned media were assayed for the production of gelatinases protein by Western blotting. Also, the inhibitory effect of the antioxidants against oxidative stress to the cells was evaluated. RESULTS: The gelatinase levels in the RPE cells increased under oxidative stress compaired with the control (p<0.05). Also, the levels decreased when the cells were incubated with the antioxidant N-acetylcysteine (NAC). CONCLUSIONS: Oxidative stress upregulates gelatinase expression in ARPE-19 cells.

Keyword

Antioxidant; Matrix metalloproteinase; Oxidative stress; Retinal pigment epithelial cell

MeSH Terms

Acetylcysteine
Antioxidants
Blotting, Western
Culture Media, Conditioned
Epithelial Cells
Gelatinases*
Humans
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Oxidative Stress*
Paraquat
Retinaldehyde
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Acetylcysteine
Antioxidants
Culture Media, Conditioned
Gelatinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Paraquat
RNA, Messenger
Retinaldehyde