Tuberc Respir Dis.  2008 Dec;65(6):504-511.

Particulate Matter 10 from Asian Dust Storms Induces the Expression of Reactive Oxygen Species, NF-kappaB, TGF-beta and Fibronectin in WI-26 VA4 Epithelial Cells

• Park KS
• Kim YJ
• Yoon JY
• Kyung SY
• An CH
• Lee SP
• Park JW
• Jeong SH

Abstract

BACKGROUND: Particulate matter may be toxic to human tissue. Ambient air particulate matter < or =10micrometer in aerodynamic size (PM10), which changes under different environmental conditions, is a complex mixture of organic and inorganic compounds. The Asian dust event caused by meteorological phenomena can also spread unique particulate matter in affected areas. We evaluated production of ROS, TGF-beta, fibronectin, and NF kappa B by exposing normal epithelial cells to Asian dust particulate matter.
METHODS
Bronchial epithelial cells were exposed to 0, 50, 100microgramg/ml of a suspension of PM10 for 24 h. ROS were detected by measurement of DCF release from DCF-DA by FACScan. TGF-beta, fibronectin, and NF kappa B were detected by western blotting.
RESULTS
PM10 exposure increased the expression of TGF-beta, fibronectin, and NF kappa B. ROS production and TGF-betalevels were significantly higher with 50 or 100microgram/ml PM10. Fibronectin and NF kappa B production were significantly higher after 100microgram/ml of PM10.
CONCLUSION
PM10 from Asian dust particles might have fibrotic potential in bronchial epithelial cells via ROS induction after PM10 exposure.

Keyword

Particulate matter; Asian dust; Reactive oxygen species; Pulmonary fibrosis

MeSH Terms

Asian Continental Ancestry Group
Blotting, Western
Dust
Epithelial Cells
Fibronectins
Humans
NF-kappa B
Particulate Matter
Pulmonary Fibrosis
Reactive Oxygen Species
Transforming Growth Factor beta
Dust
Fibronectins
NF-kappa B
Particulate Matter
Reactive Oxygen Species
Transforming Growth Factor beta

Figure

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  Figure 1 (A) ROS expression in WI26-VA4 cells after 24 hours. WI 26 cells were exposed to 0 to 100µg/ml of a suspension of ambient particulate matter with a diameter of less than 10µm (PM10). (B) ROS expression between control and NAC in WI26-VA4 cells. The ROS levels were increased after exposed to PM10, compared to those in NAC and control. ROS expression increased after exposure to PM10 in a dose-dependent manner (*p<0.05, †p<0.1).

• 

  Figure 2 NF-κB expression in WI- 26 VA4 cells after 24 hours incubation with PM10. The NF-κB Levels were increased after exposed to PM10, compared to those in NAC and control. NFκB expression increased after 24 hours of exposure to PM10 in a dose-dependent manner.

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  Figure 3 TGF-β expression in WI- 26 VA4 cells after 24 hours incubation with PM10. The TGF-β levels were increased after exposed to PM10, compared to those in NAC and control. TGF-β expression increased after 24 hours of exposure to PM10 in a dose-dependent manner (*p<0.05).

• 

  Figure 4 Fibronectin expression in WI-26 VA4 cells after 24 hours incubation with PM10. The fibronectin levels were increased after exposed to PM10, compared to those in NAC and control. fibronectin expression increased after 24 hours of exposure to PM10 in a dose-dependent manner.

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