Nutr Res Pract.
2015 Dec;9(6):586-591. 10.4162/nrp.2015.9.6.586.
Cytoprotective effect of rhamnetin on miconazole-induced H9c2 cell damage
- Affiliations
-
- 1Department of Medical Science, School of Medicine, Konkuk University, Seoul 143-701, Korea.
- 2Department of Pathology, College of Korean Medicine, Dongguk University, Gyeonggi-Do 410-820, Korea.
- 3Department of Diagnosis, College of Korean Medicine, Dongguk University, 32 Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-Do 410-820, Korea. diapwh@dongguk.ac.kr
- KMID: 2313881
- DOI: http://doi.org/10.4162/nrp.2015.9.6.586
Abstract
- BACKGROUND/OBJECTIVES
Reactive oxygen species (ROS) formation is closely related to miconazole-induced heart dysfunction. Although rhamnetin has antioxidant effects, it remained unknown whether it can protect against miconazole-induced cardiomyocyte apoptosis. Thus, we investigated the effects of rhamnetin on miconazole-stimulated H9c2 cell apoptosis.
MATERIALS/METHODS
Cell morphology was observed by inverted microscope and cell viability was determined using a WelCount(TM) cell proliferation assay kit. Miconazole-induced ROS production was evaluated by fluorescence-activated cell sorting with 6-carboxy-2',7'-dichlorofluoroscein diacetate (H2DCF-DA) stain. Immunoblot analysis was used to determine apurinic/apyrimidinic endonuclease 1 (APE/Ref-1) and cleaved cysteine-aspartic protease (caspase) 3 expression. NADPH oxidase levels were measured using real-time polymerase chain reaction.
RESULTS
Miconazole (3 and 10 microM) induced abnormal morphological changes and cell death in H9c2 cells. Rhamnetin enhanced the viability of miconazole (3 microM)-treated cells in a dose-dependent manner. Rhamnetin (1 and 3 microM) treatment downregulated cleaved caspase 3 and upregulated APE/Ref-1 expression in miconazole-stimulated cells. Additionally, rhamnetin significantly reduced ROS generation.
CONCLUSIONS
Our data suggest that rhamnetin may have cytoprotective effects in miconazole-stimulated H9c2 cardiomyocytes via ROS inhibition. This effect most likely occurs through the upregulation of APE/Ref-1 and attenuation of hydrogen peroxide levels.
Keyword
MeSH Terms
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Antioxidants
Apoptosis
Caspase 3
Cell Death
Cell Proliferation
Cell Survival
Flow Cytometry
Heart
Hydrogen Peroxide
Miconazole
Myocytes, Cardiac
NADPH Oxidase
Reactive Oxygen Species
Real-Time Polymerase Chain Reaction
Up-Regulation
Antioxidants
Caspase 3
Hydrogen Peroxide
Miconazole
NADPH Oxidase
Reactive Oxygen Species
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