Nutr Res Pract.  2011 Oct;5(5):389-395.

Protective effect of methanol extract from citrus press cakes prepared by far-infrared radiation drying on H2O2-mediated oxidative damage in Vero cells

• Wijesinghe WAJP
• Senevirathne M
• Oh MC
• Jeon YJ

Abstract

In the present study, a suitable drying method was developed for citrus press cakes (CPCs), which are produced as a by-product in citrus juice plants, and the protective effect of methanol extract of CPCs prepared by far-infrared radiation (FIR) drying against H2O2-induced DNA damage was evaluated versus that of freeze-dried CPCs. Methanol extract of FIR-dried CPCs exhibited comparatively good ROS scavenging activity versus the freeze-dried CPCs at the concentration of 100 microg/mL. The extract strongly enhanced the cell viability against H2O2-induced oxidative damage in Vero cells. Lipid peroxidation inhibitory activity of the extract from FIR-dried CPCs was comparable to that of the extract from freeze-dried CPCs. This sample also exhibited good protective effects against H2O2-mediated cell apoptosis as demonstrated by decreased apoptotic body formation in the nuclear staining with Hoechst 33342. In the comet assay, the CPC extracts exhibited strong inhibitory effects against H2O2-mediated DNA damage in a dose-dependent manner. Thus, this study demonstrated that FIR drying effectively preserves CPC as a functionally important natural antioxidant source and the FIR drying can be adapted for drying CPCs and is more economical for massive production than freeze drying.

Keyword

Antioxidant; by-product; citrus press cake; far-infrared radiation drying; flavonoid

MeSH Terms

Apoptosis
Benzimidazoles
Cell Survival
Citrus
Comet Assay
Dinucleoside Phosphates
DNA Damage
Freeze Drying
Lipid Peroxidation
Methanol
Vero Cells
Benzimidazoles
Dinucleoside Phosphates
Methanol