Nutr Res Pract.  2011 Oct;5(5):389-395.

Protective effect of methanol extract from citrus press cakes prepared by far-infrared radiation drying on H2O2-mediated oxidative damage in Vero cells

  • 1School of Marine Biomedical Sciences, Jeju National University, 1, Ara 1-dong, 102 Jejudaehak-ro, Jeju 690-756, Korea.
  • 2Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea.
  • 3Department of Food Science & Food Service Industry, Jeju International University, Jeju 690-714, Korea.


In the present study, a suitable drying method was developed for citrus press cakes (CPCs), which are produced as a by-product in citrus juice plants, and the protective effect of methanol extract of CPCs prepared by far-infrared radiation (FIR) drying against H2O2-induced DNA damage was evaluated versus that of freeze-dried CPCs. Methanol extract of FIR-dried CPCs exhibited comparatively good ROS scavenging activity versus the freeze-dried CPCs at the concentration of 100 microg/mL. The extract strongly enhanced the cell viability against H2O2-induced oxidative damage in Vero cells. Lipid peroxidation inhibitory activity of the extract from FIR-dried CPCs was comparable to that of the extract from freeze-dried CPCs. This sample also exhibited good protective effects against H2O2-mediated cell apoptosis as demonstrated by decreased apoptotic body formation in the nuclear staining with Hoechst 33342. In the comet assay, the CPC extracts exhibited strong inhibitory effects against H2O2-mediated DNA damage in a dose-dependent manner. Thus, this study demonstrated that FIR drying effectively preserves CPC as a functionally important natural antioxidant source and the FIR drying can be adapted for drying CPCs and is more economical for massive production than freeze drying.


Antioxidant; by-product; citrus press cake; far-infrared radiation drying; flavonoid

MeSH Terms

Cell Survival
Comet Assay
Dinucleoside Phosphates
DNA Damage
Freeze Drying
Lipid Peroxidation
Vero Cells
Dinucleoside Phosphates
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