J Korean Ophthalmol Soc.  2001 Jan;42(1):152-159.

Antioxidant Activity of Xanthium Strumarium L. Extracted F ractions in Lens Epithelial Cells

• Lee KW
• Lee SJ
• Park HK
• Kim MJ
• Chung YS
• Hong EK
• Joo CK

Abstract

To elucidate the antioxidant effect of Xanthium strumarium L., a fruit of Dokomari or Daekori, which is a family of chrysanthemum on the H2O2-mediated cellualr damage, we examined the effect of Xanthium strumarium L. extraction fractions on survival of human lens epithelia, HLE B-3 cells by using cell culture system. H2O2-mediated cellualr death and its IC5 0, with approximately 100 micrometer H2O2 were determined by using MTT assay. The HLE B-3 cells pretreated with Xanthium strumarium L. extract fractions, were incubated with 100 micrometer H2O2, and in order to assess the cell viability the cultures were incubated with MTT solution. Among Xanthium strumarium L. extract fractions, Xan crude fraction, Xan CHCl3 fraction, and Xan EtAc fraction have antioxidant activity at the concentrations of 500 ng/ml, 1 microgram/ml and 100 ng/ml, respectively. These effects were statistically significant(p<0.05). Also, the fact that only the frations extracted with organic solvents have antioxidant activity suggests that the active components from the extract have hydrophobic property.

Keyword

Cataract; Lens epithelial cells; Antioxidant; Oxidative stress

MeSH Terms

Antioxidants
Cataract
Cell Culture Techniques
Cell Survival
Chrysanthemum
Epithelial Cells*
Fruit
Humans
Oxidative Stress
Solvents
Xanthium*
Antioxidants
Solvents