J Korean Ophthalmol Soc.
2002 Apr;43(4):751-756.
Antioxidant Assay of Extracted Fractions Xanthium Strumarium L Using Lens Protein Crosslink Activity
- Affiliations
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- 1Laboratory of Ophthalmology and Visual Science, College of Medicine, The Catholic university of Korea, Seoul, Korea. ckjoo@cmc.cuk.ac.kr
- 2Medvill.Co.Ltd, Korea.
- 3Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
Abstract
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PURPOSE: To elucidate the anti-oxidant effect of extract fractions from Xanthium strumarium L. on lens protein by crosslinking assay.
METHODS
[(1 4)C] N-formyl-lysine was synthesized and purified by ion exchange chromatography. The crosslinking activities of extract fractions(Xan Crude, Xan CHCl3, Xan EtAc and Xan H2O) to lens protein were determined by incorporation with [(14)C] N-formyl-lysine.
RESULTS
It was observed that Xan Crude, Xan CHCl3, and Xan EtAc extracted from Xanthium strumarium L. showed approximately 10% of antioxidant effect whereas Xan H2O showed no effect by crosslinking assay.
CONCLUSIONS
This study showed that the crosslinking assay described in this study can be developed as a potential tool to screen the anti-oxidant effect rapidly and accurately compared to MTT assay. The result was compared to MTT assay using Human Lens epithelial cell line.
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