J Adv Prosthodont.  2016 Jun;8(3):172-180. 10.4047/jap.2016.8.3.172.

Antimicrobial, Antioxidant and Cytotoxic Activities of Dendropanax morbifera Léveille extract for mouthwash and denture cleaning solution

• Kim RW
• Lee SY
• Kim SG
• Heo YR
• Son MK

• KMID: 2376840
• DOI: http://doi.org/10.4047/jap.2016.8.3.172

Abstract

PURPOSE
The purpose of this study was to analyze the antimicrobial, antioxidant activity and cytotoxicity of Dendropanax morbifera Léveille extract for assessing whether Dendropanax morbifera Léveille can be used for the development of natural mouthwash and denture cleaning solution.
MATERIALS AND METHODS
The extract was obtained from branches of Dendropanax morbifera Léveille. The solvent fractions were acquired by fractionating Dendropanax morbifera Léveille extract using n-hexane, ethyl acetate, chloroform and butanol solvent. Paper disc test was used to evaluate the antimicrobial and antifungal activity of Dendropanax morbifera Léveille extract and solvent fractions against Streptococcus mutans and Candida albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of Dendropanax morbifera Léveille extract was analyzed through MTT assay using normal human oral keratinocytes.
RESULTS
Dendropanax morbifera Léveille extract showed antimicrobial activity against Streptococcus mutans and especially Candida albicans. The solvent fractions of Dendropanax morbifera Léveille showed strong antimicrobial activity against Streptococcus mutans and Candida albicans in n-hexane and butanol solvent fraction, respectively. Dendropanax morbifera Léveille extract also showed outstanding antioxidant activity. Butanol, ethyl acetate, and chloroform solvent fraction of Dendropanax morbifera Léveille tended to have increased antioxidant activity as the concentration increased. Dendropanax morbifera Léveille extract showed high cell survival rate in cytotoxicity test.
CONCLUSION
Dendropanax morbifera Léveille extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that Dendropanax morbifera Léveille is applicable as an ingredient for natural mouthwash and denture cleanser.

Keyword

Dendropanax morbifera Léveille; Antimicrobial activity; Antioxidant activity; Cytotoxicity; Denture cleaning solution

MeSH Terms

Candida albicans
Cell Survival
Chloroform
Denture Cleansers
Dentures*
Humans
Keratinocytes
Streptococcus mutans
Chloroform
Denture Cleansers

Figure

• 

  Fig. 1 Antibacterial and antifungal activity of Dendropanax morbifera Léveille extract against (A) S.mutans and (B) C. albicans (C; Control, 20; 20 µg/mL, 40; 40 µg/mL, 80; 80 µg/mL, 100; 100 µg/mL).

• 

  Fig. 2 Antibacterial activity of the Dendropanax morbifera Léveille solvent fraction against S. mutans (C; Control, 20; 20 µg/mL, 40; 40 µg/mL, 80; 80 µg/mL, 100; 100 µg/mL). (A) n-hexane, (B) ethyl acetate, (C) chloroform, (D) butanol.

• 

  Fig. 3 Antifungal activity of the Dendropanax morbifera Léveille solvent fraction against C. albicans (C; Control, 20; 20 µg/mL, 40; 40 µg/mL, 80; 80 µg/mL, 100; 100 µg/mL). (A) n-hexane, (B) ethyl acetate, (C) chloroform, (D) butanol.

• 

  Fig. 4 Cell viability of the Dendropanax morbifera Léveille extracts.

Reference

1. Glass RT, Conrad RS, Bullard JW, Goodson LB, Mehta N, Lech SJ, Loewy ZG. Evaluation of microbial flora found in previously worn prostheses from the Northeast and Southwest regions of the United States. J Prosthet Dent. 2010; 103:384–389. PMID: 20493328.

2. Budtz-Jörgensen E. The significance of Candida albicans in denture stomatitis. Scand J Dent Res. 1974; 82:151–190. PMID: 4598186.

3. Baena-Monroy T, Moreno-Maldonado V, Franco-Martínez F, Aldape-Barrios B, Quindós G, Sánchez-Vargas LO. Candida albicans, Staphylococcus aureus and Streptococcus mutans colonization in patients wearing dental prosthesis. Med Oral Patol Oral Cir Bucal. 2005; 10:E27–E39. PMID: 15800465.

4. Campos MS, Marchini L, Bernardes LA, Paulino LC, Nobrega FG. Biofilm microbial communities of denture stomatitis. Oral Microbiol Immunol. 2008; 23:419–424. PMID: 18793366.

5. Ramage G, Tomsett K, Wickes BL, López-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 98:53–59. PMID: 15243471.

6. Caton JG, Blieden TM, Lowenguth RA, Frantz BJ, Wagener CJ, Doblin JM, Stein SH, Proskin HM. Comparison between mechanical cleaning and an antimicrobial rinse for the treatment and prevention of interdental gingivitis. J Clin Periodontol. 1993; 20:172–178. PMID: 8450082.

7. Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod. 2003; 66:1022–1037. PMID: 12880330.

8. Lee SH, Park YS, Hwang B, Kim JH, Lee HY. Screening of immune activation activities in the Leaves of Dendropanax morbifera Lev. Korean J Med Crop Sci. 2002; 10:109–115.

9. Hyun TK, Kim MO, Lee H, Kim Y, Kim E, Kim JS. Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Léveille. Food Chem. 2013; 141:1947–1955. PMID: 23870914.

10. Moon HI. Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats. Hum Exp Toxicol. 2011; 30:870–875. PMID: 20716587.

11. Chung IM, Kim MY, Park WH, Moon HI. Antiatherogenic activity of Dendropanax morbifera essential oil in rats. Pharmazie. 2009; 64:547–549. PMID: 19746846.

12. Chung IM, Song HK, Kim SJ, Moon HI. Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille. Phytother Res. 2011; 25:784–786. PMID: 21520473.

13. Lee CK, Kim H, Moon KH, Shin KH. Screening and isolation of antibiotic resistance inhibitors from herb materials-resistance inhibition of volatile components of Korean aromatic herbs. Arch Pharm Res. 1998; 21:62–66. PMID: 9875516.

14. Park YK, Lee WY, Ahn JK. Current review on the study of antioxidants developments from forest resources. Trend Agric Life Sci. 2006; 4:1–13.

15. Wikins TD, Holdeman LV, Abramson IJ, Moore WE. Standardized single-disc method for antibiotic susceptibility testing of anaerobic bacteria. Antimicrob Agents Chemother. 1972; 1:451–459. PMID: 4680809.

16. Brand-Williams W, Cuvelier M, Berset C. Use of a free radical method to evaluate antioxidant activity. Technol. 1995; 28:25–30.

17. Pratten J, Smith AW, Wilson M. Response of single species biofilms and microcosm dental plaques to pulsing with chlorhexidine. J Antimicrob Chemother. 1998; 42:453–459. PMID: 9818743.

18. Gjermo P. Chlorhexidine and related-compounds. J Dent Res. 1989; 68:1602–1608.

19. Kim H, Song MJ. Analysis and recordings of orally transmitted knowledge about medicinal plants in the southern mountainous region of Korea. J Ethnopharmacol. 2011; 134:676–696. PMID: 21256949.

20. Islam B, Khan SN, Khan AU. Dental caries: from infection to prevention. Med Sci Monit. 2007; 13:RA196–RA203. PMID: 17968308.

21. Kulak Y, Arikan A, Kazazoglu E. Existence of Candida albicans and microorganisms in denture stomatitis patients. J Oral Rehabil. 1997; 24:788–790. PMID: 9372471.

22. Im GP, Jeong HJ, Lee JS. Studies on the Technical Improvement to use "Hwangchil-traditional Korean golden varnish" and the diversified uses of Dendropanax morbiera Lev.2nd Report. Korea: Korean Ministry of Science and Technology;1996. p. 1–257.

23. Baek DH. Screening of the natural plant extracts for the antimicrobial activity on dental pathogens. Korean J Microbiol. 2007; 43:227–231.

24. Otake S, Makimura M, Kuroki T, Nishihara Y, Hirasawa M. Anticaries effects of polyphenolic compounds from Japanese green tea. Caries Res. 1991; 25:438–443. PMID: 1667297.

25. Smullen J, Koutsou GA, Foster HA, Zumbé A, Storey DM. The antibacterial activity of plant extracts containing polyphenols against Streptococcus mutans. Caries Res. 2007; 41:342–349. PMID: 17713333.

26. Kim HR, Chung HJ. Chemical characteristics of the leaves and the seeds of Korean Dendropanax (Dendropanax morbifera Lev.). J Korean Soc Appl Biol Chem. 2000; 43:63–66.

27. Yanagida A, Kanda T, Tanabe M, Matsudaira F, Oliveira Cordeiro JG. Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutans streptococci. J Agric Food Chem. 2000; 48:5666–5671. PMID: 11087536.

28. Son HY, Kim EJ, Gwon YS, Gwon GS, Jin IR, Gwon HY, Gwon JS, Son GH. Screening of anti-candidiosis agent from medicinal and wild plants. J Life Sci. 2003; 13:604–617.

29. Coleman JJ, Okoli I, Tegos GP, Holson EB, Wagner FF, Hamblin MR, Mylonakis E. Characterization of plant-derived saponin natural products against Candida albicans. ACS Chem Biol. 2010; 5:321–332. PMID: 20099897.

30. Zou Y, Liao S, Shen W, Liu F, Tang C, Chen CY, Sun Y. Phenolics and antioxidant activity of mulberry leaves depend on cultivar and harvest month in Southern China. Int J Mol Sci. 2012; 13:16544–16553. PMID: 23443117.

31. Hong S, Jeong D, Kim K, Hwang E. The composition of the root of Ixeris dentate var. albiflora Nakai. and cell viability and DPPH radical scavenging activities of tist extract. Korean J Nutr. 2010; 43:105–113.

32. Shin IC, Jeong KJ, Shim TH, Oh HS, Park SK, Cheung EH, Kim SN, Kim GG, Choi DS, Kwon YS, Kim CM, Sa JH. Catechin content and antioxidative effect from Rosa davurica Pall. Korean J Pharmacogn. 2002; 33:177–181.

33. Jang JR, Hwang SY, Lim SY. Inhibitory effect of extracts of Platycodon grandiflorum (the ballon flower) on oxidation and nitric oxide production. Korean J Food Preserv. 2011; 18:65–71.

34. Helgeland K, Heyden G, Rölla G. Effect of chlorhexidine on animal cells in vitro. Scand J Dent Res. 1971; 79:209–215. PMID: 4105986.

35. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987; 47:936–942. PMID: 3802100.

36. Park SA, Park J, Park CI, Jie YJ, Hwang YC, Kim YH, Jeon SH, Lee HM, Ha JH, Kim KJ, Park SM. Cellular antioxidant activity and whitening effects of Dendropanax morbifera leaf extracts. Korean J Microbiol Biotechnol. 2013; 41:407–415.