J Adv Prosthodont.  2016 Jun;8(3):172-180. 10.4047/jap.2016.8.3.172.

Antimicrobial, Antioxidant and Cytotoxic Activities of Dendropanax morbifera Léveille extract for mouthwash and denture cleaning solution

Affiliations
  • 1Department of Prosthodontics, School of Dentistry, Chosun University, Gwangju, Republic of Korea. son0513@chosun.ac.kr
  • 2Regional Innovation Center for Dental Science & Engineering, Chosun University, Gwangju, Republic of Korea.
  • 3Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

Abstract

PURPOSE
The purpose of this study was to analyze the antimicrobial, antioxidant activity and cytotoxicity of Dendropanax morbifera Léveille extract for assessing whether Dendropanax morbifera Léveille can be used for the development of natural mouthwash and denture cleaning solution.
MATERIALS AND METHODS
The extract was obtained from branches of Dendropanax morbifera Léveille. The solvent fractions were acquired by fractionating Dendropanax morbifera Léveille extract using n-hexane, ethyl acetate, chloroform and butanol solvent. Paper disc test was used to evaluate the antimicrobial and antifungal activity of Dendropanax morbifera Léveille extract and solvent fractions against Streptococcus mutans and Candida albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of Dendropanax morbifera Léveille extract was analyzed through MTT assay using normal human oral keratinocytes.
RESULTS
Dendropanax morbifera Léveille extract showed antimicrobial activity against Streptococcus mutans and especially Candida albicans. The solvent fractions of Dendropanax morbifera Léveille showed strong antimicrobial activity against Streptococcus mutans and Candida albicans in n-hexane and butanol solvent fraction, respectively. Dendropanax morbifera Léveille extract also showed outstanding antioxidant activity. Butanol, ethyl acetate, and chloroform solvent fraction of Dendropanax morbifera Léveille tended to have increased antioxidant activity as the concentration increased. Dendropanax morbifera Léveille extract showed high cell survival rate in cytotoxicity test.
CONCLUSION
Dendropanax morbifera Léveille extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that Dendropanax morbifera Léveille is applicable as an ingredient for natural mouthwash and denture cleanser.

Keyword

Dendropanax morbifera Léveille; Antimicrobial activity; Antioxidant activity; Cytotoxicity; Denture cleaning solution

MeSH Terms

Candida albicans
Cell Survival
Chloroform
Denture Cleansers
Dentures*
Humans
Keratinocytes
Streptococcus mutans
Chloroform
Denture Cleansers

Figure

  • Fig. 1 Antibacterial and antifungal activity of Dendropanax morbifera Léveille extract against (A) S.mutans and (B) C. albicans (C; Control, 20; 20 µg/mL, 40; 40 µg/mL, 80; 80 µg/mL, 100; 100 µg/mL).

  • Fig. 2 Antibacterial activity of the Dendropanax morbifera Léveille solvent fraction against S. mutans (C; Control, 20; 20 µg/mL, 40; 40 µg/mL, 80; 80 µg/mL, 100; 100 µg/mL). (A) n-hexane, (B) ethyl acetate, (C) chloroform, (D) butanol.

  • Fig. 3 Antifungal activity of the Dendropanax morbifera Léveille solvent fraction against C. albicans (C; Control, 20; 20 µg/mL, 40; 40 µg/mL, 80; 80 µg/mL, 100; 100 µg/mL). (A) n-hexane, (B) ethyl acetate, (C) chloroform, (D) butanol.

  • Fig. 4 Cell viability of the Dendropanax morbifera Léveille extracts.


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