J Korean Ophthalmol Soc.  2003 Jan;44(1):195-201.

The changes of fibronectin in the cultured porcine lens epithelial cells

• Hong SB
• Lee JH
• Kim MJ
• Oh JH

Abstract

PURPOSE
to investigate change of fibronectin as a relating factor of cellular migration in the cultured lens epithelial cells. METHODS: We attempted to observe the fibronectin from cornea, crystalline lens and cultured lens epithelial cells by immunocytochemistry. We also compared the expression of fibronectin from normal porcine crystalline lens epithelial cells and cultured lens epithelial cells that received bFGF with western blot method. RESULTS: Fibronectin was detected in the extracellular matrix of normal corneal epithelium by immunocytochemistry, but not in the normal lens epithelial cells. However, fibronectin was detected in the cytoplasm of the cultured lens epithelial cells and treated with bFGF by immunocytochemistry. CONCLUSIONS: lens epithelial cells in normal condition may not produce fibronectin, but lens epithelial cells without lens nucleus after cataract surgery produce fibronectin.

Keyword

bFGF (basic fibroblast growth factor); Cataract; Fibronectin; Lens epithelial cells

MeSH Terms

Blotting, Western
Cataract
Cornea
Cytoplasm
Epithelial Cells*
Epithelium, Corneal
Extracellular Matrix
Fibronectins*
Immunohistochemistry
Lens, Crystalline
Fibronectins