Korean J Physiol Pharmacol.  2014 Aug;18(4):289-296. 10.4196/kjpp.2014.18.4.289.

Effect of Phorbol 12-Myristate 13-Acetate on the Differentiation of Adipose-Derived Stromal Cells from Different Subcutaneous Adipose Tissue Depots

Affiliations
  • 1Aesthetic, Plastic, & Reconstructive Surgery Center, Good Moonhwa Hospital, Busan 614-847, Korea.
  • 2S&M Research Institute, Good Moonhwa Hospital, Busan 614-847, Korea.
  • 3Center for Reproductive Medicine, Good Moonhwa Hospital, Busan 614-847, Korea.
  • 4Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-790, Korea. jsjung@pusan.ac.kr

Abstract

Human adipose-tissue-derived stromal cells (hADSCs) are abundant in adipose tissue and can differentiate into multi-lineage cell types, including adipocytes, osteoblasts, and chondrocytes. In order to define the optimal harvest site of adipose tissue harvest site, we solated hADSCs from different subcutaneous sites (upper abdomen, lower abdomen, and thigh) and compared their proliferation and potential to differentiate into adipocytes and osteoblasts. In addition, this study examined the effect of phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, on proliferation and differentiation of hADSCs to adipocytes or osteoblasts. hADSCs isolated from different subcutaneous depots have a similar growth rate. Fluorescence-activated cell sorting (FACS) analysis showed that the expression levels of CD73 and CD90 were similar between hADSCs from abdomen and thigh regions. However, the expression of CD105 was lower in hADSCs from the thigh than in those from the abdomen. Although the adipogenic differentiation potential of hADSCs from both tissue regions was similar, the osteogenic differentiation potential of hADSCs from the thigh was greater than that of hADSCs from the abdomen. Phorbol 12-myristate 13-acetate (PMA) treatment increased osteogenic differentiation and suppressed adipogenic differentiation of all hADSCs without affecting their growth rate and the treatment of Go6983, a general inhibitor of protein kinase C (PKC) blocked the PMA effect. These findings indicate that the thigh region might be a suitable source of hADSCs for bone regeneration and that the PKC signaling pathway may be involved in the adipogenic and osteogenic differentiation of hADSCs.

Keyword

Differentiation; hADSCs; PKC; pPMA; Proliferation

MeSH Terms

Abdomen
Adipocytes
Adipose Tissue
Bone Regeneration
Chondrocytes
Flow Cytometry
Humans
Osteoblasts
Protein Kinase C
Stromal Cells*
Subcutaneous Fat*
Thigh
Protein Kinase C
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