Korean J Physiol Pharmacol.  2012 Dec;16(6):399-404. 10.4196/kjpp.2012.16.6.399.

Inhibitory Effects of ECQ on Indomethacin-Induced Gastric Damage in Rats

• Jung J
• Nam Y
• Sohn UD

• KMID: 2285448
• DOI: http://doi.org/10.4196/kjpp.2012.16.6.399

Abstract

We investigated inhibitory effects of extract containing quercetin-3-O-beta-D-glucuronopyranoside (ECQ) extracted from Rumex Aquaticus Herba on indomethacin-induced gastric damage in Rats. Gastritis was induced in male Sprague-Dawley rats (200~220 g) by oral administration of indomethacin at a dose of 40 mg/kg. One hour before administration of indomethacin, animals were orally pretreated with ECQ at doses of 0.3, 1, 3 or 10 mg/kg. Six hours after indomethacin administration, the rats were sacrificed and the stomach was excised and opened along the greater curvature, and the surface area of gastric lesion was measured using optical microscope. Superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) activities and malondialdehyde (MDA) levels were measured by ELISA. Western blot analysis was performed to detect protein expression of SOD-2. Linear hemorrhagic mucosal lesions were observed in the stomach 6 hours after oral administration of indomethacin. Pretreatment with ECQ significantly reduced the severity of the lesions in a dose-dependent manner. It also inhibited the reductions in SOD and CAT activities and SOD expression by the indomethacin-induced gastric damage. In addition, the pretreatment with ECQ significantly suppressed the elevation of the MPO activity and the MDA levels induced by indomethacin. These results suggest that ECQ has the inhibitory effects via antioxidative action against indomethacin-induced gastritis in rats.

Keyword

Extract; Flavonoid; Indomethacin-induced gastritis; QGC; Rats

MeSH Terms

Administration, Oral
Animals
Blotting, Western
Catalase
Cats
Enzyme-Linked Immunosorbent Assay
Gastritis
Humans
Indomethacin
Male
Malondialdehyde
Peroxidase
Quercetin
Rats
Rats, Sprague-Dawley
Rumex
Stomach
Superoxide Dismutase
Catalase
Indomethacin
Malondialdehyde
Peroxidase
Quercetin
Superoxide Dismutase

Figure

• 

  Fig. 1 Protective Effect of ECQ on gastric lesions induced by indomethacin (IND) in rats. (A) Representative macroscopic findings of the gastric lesions induced by IND 40 mg/kg in rats: (a) Vehicle group, (b) IND group, (c~f) One hour before oral administration of IND 40 mg/kg, animals were orally pretreated with ECQ at dose of 0.3, 1.0, 3.0, or 10 mg/kg, respectively. Six hours later, the animals were then sacrificed by cervical dislocation and the stomach was harvested. (B) Macroscopic damage was quantified by measuring the extent of the gastric mucosal lesions. Each value represents the mean±S.E.M. (n=5 per group). *p<0.01 and **p<0.001 vs. IND group.

• 

  Fig. 2 Effect of ECQ on indomethacin (IND)-induced gastric mucosal SOD activity in rats. ECQ (0.3, 1.0, 3.0, or 10 mg/kg) were pretreated one hour before administration of IND, and then SOD activity was estimated 6 hours after the IND administration. Each value represents the mean±S.E.M. (n=5 per group). *p<0.001 vs. vehicle group, #p<0.05 vs. IND group.

• 

  Fig. 3 Effect of ECQ on indomethacin (IND)-induced gastric mucosal CAT activity in rats. ECQ (0.3, 1.0, 3.0, or 10 mg/kg) were given one hour before administration of IND, and then CAT activity was estimated 6 hours after the IND administration. Each value represents the mean±S.E.M. (n=5 per group). *p<0.05 vs. vehicle group, #p<0.01 vs. IND group.

• 

  Fig. 4 The expression of SOD-2 in indomethacin (IND) treated groups with or without ECQ. ECQ (0.3, 1.0, 3.0, or 10 mg/kg) were pretreated one hour before administration of IND, and then SOD-2 expression was detected by Western blot analysis 6 hours after the IND administration (The representative blot is shown). Each value represents the mean±S.E.M. (n=5 per group). *p<0.05 vs. vehicle group, #p<0.05 vs. IND group.

• 

  Fig. 5 Effect of ECQ on indomethacin (IND)-induced gastric MPO activity in rats. ECQ (0.3, 1.0, 3.0, or 10 mg/kg) were pretreated one hour before administration of IND, and then MPO activity was estimated 6 hours after the IND administration. Each value represents the mean±S.E.M. (n=5 per group). *p<0.05 vs. vehicle group, #p<0.05 vs. IND group.

• 

  Fig. 6 Effect of ECQ on indomethacin (IND)-induced increases in gastric MDA level in rats. ECQ (0.3, 1.0, 3.0, or 10 mg/kg) were pretreated one hour before IND administration, and then MDA level was estimated 6 hours after the IND administration. Each value represents the mean±S.E.M. (n=5 per group). *p<0.05 vs. vehicle group, #p<0.01 vs. IND group.

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