Korean J Phys Anthropol.  1999 Dec;12(2):297-303.

Immunohistochemical Expression of Inducible Nitric Oxide Synthase on the LPS-induced Shock and Wound Healing in Rats

• Choi BT
• Ko WS
• Lee YT
• Kim GC
• Lee JH
• Gil YG

Abstract

Inducible nitric oxide synthase (iNOS) expression of several organs on the lipopolysaccharides (LPS)-injected rats and on excisional wound was observed by immunohistochemical methods to investigate iNOS-positive cells during inflammation. iNOS expression was induced in response to LPS in the brain and these reactions were observed in the choroidal epithelium, ependymal cells and a few of nerve cells and fiber. A more intensive reaction of nerve cell and fiber was mainly observed in the corpus callosum and hypothalamus. Induction of iNOS of the lung was observed in alveolar macrophage, smooth muscle, pneumocytes and inflammatory cells infilterated in the alveolar septum. iNOS expression of the liver was detected in Kupffer cells, hepatocytes, bile duct and inflammatory cells of spotty necrosis. The cardiac muscle and endothelial cell of the heart showed positive iNOS expression. In the excisional wound, inflammatory cells including macrophages, neutrophil and fibrobast showed iNOS expression and mainly detected necrobiotic layer. Collectively, iNOS expression was induced in the several cell types during inflammatory process. So for better understanding the function of iNOS, more research should be done in relation to each cell type of organ.

Keyword

iNOS; LPS; Wound; Inflammation

MeSH Terms

Animals
Bile Ducts
Brain
Choroid
Corpus Callosum
Endothelial Cells
Epithelium
Heart
Hepatocytes
Hypothalamus
Inflammation
Kupffer Cells
Lipopolysaccharides
Liver
Lung
Macrophages
Macrophages, Alveolar
Muscle, Smooth
Myocardium
Necrosis
Neurons
Neutrophils
Nitric Oxide Synthase Type II*
Pneumocytes
Rats*
Shock*
Wound Healing*
Wounds and Injuries*
Lipopolysaccharides
Nitric Oxide Synthase Type II