Korean J Pathol.  2011 Feb;45(1):1-8.

Effect of Selective Cyclooxygenase 2 Inhibitor in TCDD Pre-exposed Thyroid Papillary Carcinoma Cell Line

• Kim HS
• Ahn KS
• Lee JH
• Chae YS
• Won NH
• Choi JS
• Kim CH

Abstract

BACKGROUND
Cyclooxygenase 2 (COX-2) is related to carcinogenesis and progression of cancer. COX-2 has been detected in thyroid cancer. This suggests that COX-2 inhibitor may be useful to control the growth of thyroid cancer cells as well as the progression of thyroid cancer. Tetrachlorodibenzodioxin (TCDD), acting as an inflammatory cytokine, directly induces the expression of COX-2. We examine whether TCDD controls the effect of COX-2 inhibitor on thyroid cancer cells.
METHODS
The effects of TCDD and celecoxib on thyroid papillary carcinoma cell line (SNU790) were examined using cell proliferation and fluorescence-activated cell sorting analysis. Western blot analysis was performed to determine the expressed COX-2 levels and the cell cycle-related proteins. The matrix metalloproteinase-2 (MMP-2) expression and gelatinolytic activity were examined using real time-polymerase chain reaction and zymography.
RESULTS
TCDD directly induced the growth of SNU790 and the expression of cyclin D1, cyclin A, cyclin E, p21 and COX-2. Celecoxib suppressed the growth of SNU790 and the expression of cyclin D1 and cyclin E. Celecoxib reduced the MMP-2 expression and the gelatinolytic activity, but those effects were decreased in the SNU790 by either pre-treatment with TCDD or co-treatment with TCDD and celecoxib.
CONCLUSIONS
Celocoxib effect is directly reduced depending on the exposure to TCDD. TCDD exposure should be considered in the treatment with Celecoxib.

Keyword

Cyclooxygenase 2; Cyclooxygenase 2 inhibitors; Tetrachlorodibenzodioxin; Carcinoma, papillary; Thyroid gland

MeSH Terms

Blotting, Western
Carcinoma, Papillary
Cell Line
Cell Proliferation
Cyclin A
Cyclin D1
Cyclin E
Cyclins
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Flow Cytometry
Matrix Metalloproteinase 2
Prostaglandin-Endoperoxide Synthases
Proteins
Pyrazoles
Sulfonamides
Tetrachlorodibenzodioxin
Thyroid Gland
Thyroid Neoplasms
Celecoxib
Cyclin A
Cyclin D1
Cyclin E
Cyclins
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Matrix Metalloproteinase 2
Prostaglandin-Endoperoxide Synthases
Proteins
Pyrazoles
Sulfonamides
Tetrachlorodibenzodioxin