Korean J Otorhinolaryngol-Head Neck Surg.  2009 Jun;52(6):498-505. 10.3342/kjorl-hns.2009.52.6.498.

The Effect of Asian Sand Dust in Allergic Inflammation of Allergic Mouse

• Kim ST
• Lee EJ
• Jung JH
• Gang IG
• Cha HE
• Kim DY
• Do SH

• KMID: 2277065
• DOI: http://doi.org/10.3342/kjorl-hns.2009.52.6.498

Abstract

BACKGROUND AND OBJECTIVES: Asian sand dust (ASD) is known to aggravate the respiratory symptoms in patients with bronchial asthma. However, the effect of ASD in allergic rhinitis is not known. The objective of this study was to investigate whether ASD can activate the allergic inflammation in allergic mouse model. MATERIALS AND METHOD: Forty female BALB/c mice were divided into 4 groups. Group 1 was nebulized with saline and group 2 with ASD. Group 3 was nebulized with ovalbumin (OVA) only and Group 4 with OVA plus ASD after intraperitoneal injection with OVA. The allergic symptom scores were checked. The mouse OVA specific IgE/IgG1, IL-4, IL-5 and IFN-gamma were measured by ELISA. The nasal mucosa was examined for the expression of IL-4 and IL-5 by immunohistochemical stain.
RESULTS
The average symptom score was increased in Group 4 compared to Group 3 (p< 0.05). The IgE was significantly increased in Group 4 compared to Group 3 (p< 0.01). The IL-4 level of nasal lavagefluid (NALF) was significantly increased in Group 4 compared to Group 3 (p< 0.05). The IL-5 level showed no significant difference between the Group 3 and Group 4 both in the serum and NALF. The level of IFN-gamma was not changed in NALF. Immunohistochemical staining showed that the positive cells for IL-4 were expressed in epithelial layer and submucous gland and the positive cells were more increased in Group 4 than in Group 3.
CONCLUSION
In allergic mouse model, ASD has shown to activate the allergic inflammatory reaction by the stimuli of Th2 cytokineproduction.

Keyword

Asian sand dust; Allergic rhinitis; Cytokines

MeSH Terms

Animals
Asian Continental Ancestry Group
Asthma
Cytokines
Dust
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin E
Inflammation
Injections, Intraperitoneal
Interleukin-4
Interleukin-5
Mice
Nasal Mucosa
Ovalbumin
Ovum
Rhinitis
Rhinitis, Allergic, Perennial
Silicon Dioxide
Cytokines
Dust
Immunoglobulin E
Interleukin-4
Interleukin-5
Ovalbumin
Silicon Dioxide