Korean J Otorhinolaryngol-Head Neck Surg.
2008 Jan;51(1):46-50.
Interleukin-1beta Decreases Caveolin-1 Expression in Human Nasal Epithelium
- Affiliations
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- 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Konyang University, Daejeon, Korea.
- 2Department of Otorhinolaryngology-Head and Neck Surgery, Inha University Hospital, Incheon, Korea. jangty@inha.ac.kr
- 3Younsei Coen ENT Clinic, Seoul, Korea.
- 4Department of Pharmacology, Center for Advanced Medical Education, Inha University College of Medicine, Incheon, Korea.
Abstract
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BACKGROUND AND OBJECTIVES: Caveolin-1 (Cav-1) is the structural protein that is necessary for the formation of caveolae membrane domains. It is known as an inhibitor of various signaling pathways and associated with several diseases such as cancer, atherosclerosis, restrictive lung disease and obesity. However, studies for Cav-1 in nose has been hardly performed. The objectives of our study were to detect Cav-1 expression in human nasal epithelium and to investigate the change of Cav-1 expression in the inflammation of nasal epithelium.
SUBJECTS AND METHOD
We obtained nasal polyp specimens from three patients undergoing endoscopic sinus surgery. Cells from specimens were cultured using the air-liquid interface technique and IL-1beta was treated. The expression of Cav-1 mRNA and protein was determined by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis, respectively.
RESULTS
Both RT-PCR and Western blot analysis demonstrated the presence of Cav-1 mRNA and protein in human nasal epithe-lium. Furthermore, the expression of both Cav-1 mRNA and protein was decreased by IL-1beta stimulation.
CONCLUSION
Cav-1 was expressed in human nasal epithelial cells. It is assumed that Cav-1 may play a role in nasal inflammatory disease. However, further studies to confirm the interaction between Cav-1 and signaling molecules in the nasal inflammatory process should be followed.
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