Korean J Otolaryngol-Head Neck Surg.  2002 Feb;45(2):132-136.

IL-1beta Mediated COX-2 Expression in Human Airway Epithelial Cells

Affiliations
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Taegu, Korea. ydkim@med.yu.ac.kr
  • 2Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Taegu, Korea.

Abstract

BACKGROUND AND OBJECTIVES: Prostaglandin is one of the important inflammatory mediator in inflammatory diseases. Cyclooxygenase-2 (COX-2) plays a key role in biosynthesis of prostaglandins. In this study, we aimed to investigate COX-2 expression and prostaglandin E2 (PGE2) production by interleukin-1beta (IL-1beta) in cultured human airway epithelial cells. MATERIALS AND METHOD: COX-2 gene expression, and COX-2 protein, PGE2 production by IL-1beta were analyzed by RT-PCR, Western blot, and enzymeimmunoassay (EIA) in cultured human airway NCI-H292 epithelial cells.
RESULTS
The COX-2 protein production was increased when the cells were exposed to IL-1beta in a dose dependent manner. The maximum level of COX-2 protein was detected at 20 ng/ml of IL-1beta. After 4 hours, the production of COX-2 protein was detected by IL-1beta(20 ng/ml) and this was held up to 12 hour. The maximum level of COX-2 protein production reached at 8 hour of exposure to IL-1beta and this was held up to 12 hour. The release of PGE2 occurred in the same pattern as the IL-1beta-mediated COX-2 protein production. The COX-2 gene expression was induced by IL-1beta (20 ng/ml). The IL-1beta-mediated COX-2 expression was suppressed by actinomycin D, but was not affected by cycloheximide.
CONCLUSION
These results suggest that the IL-1beta-mediated COX-2 expression and the PGE2 production were increased in dose and time dependent manner and regulated in the transcriptional step.

Keyword

Interleukin-1beta; Cyclooxygenase-2; Prostaglandin E2; NCI-H292 cells

MeSH Terms

Blotting, Western
Cycloheximide
Cyclooxygenase 2
Dactinomycin
Dinoprostone
Epithelial Cells*
Gene Expression
Humans*
Interleukin-1beta
Prostaglandins
Prostaglandins I
Cycloheximide
Cyclooxygenase 2
Dactinomycin
Dinoprostone
Interleukin-1beta
Prostaglandins
Prostaglandins I
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