Korean J Otolaryngol-Head Neck Surg.  2004 Oct;47(10):988-993.

Effects of Anti-Inflammatory Drugs on the Interleukin-1 beta-Induced Cyclooxygenase-2 Expression in Human Airway Epithelial Cells

• Kim YD
• Woo HJ
• Bai CH
• Sin JH
• Seo YJ
• Park KS
• Song SY

Abstract

BACKGROUND AND OBJECTIVES
Cyclooxygenase-2 (COX-2) is a key molecule in the biosynthesis of prostaglandins, which are important inflammatory mediators in human airway inflammatory diseases. This study was designed to investigate the effects of several COX inhibitors on the interleukin-1beta (IL-1beta)-mediated COX-2 expression in human airway epithelial cells. MATERIALS AND METHOD: We observed the effects of anti-inflammatory drugs such as budesonide, triamcinolone, dexamethasone, NS-398, indomethacin, salicylate and resveratrol on the IL-1beta-induced COX-2 expression in cultured human airway NCI-H292 epithelial cells. The levels of COX-2 mRNA and COX-2 protein were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.
RESULTS
NS398, reveratrol and three corticosteroids strongly suppressed the IL-1beta-mediated COX-2 expression. However, indomethacin and salicylate did not inhibit or inhibited only weakly.
CONCLUSION
The extent of IL-1beta-induced supression of COX-2 expression in the cultured human airway NCI-H292 epithelial cells depended on the kinds of anti-inflammatory drugs.

Keyword

Cyclooxygenase; Interleukin-1; Inhibitor

MeSH Terms

Adrenal Cortex Hormones
Blotting, Western
Budesonide
Cyclooxygenase 2*
Dexamethasone
Epithelial Cells*
Humans*
Indomethacin
Interleukin-1*
Interleukin-1beta
Prostaglandin-Endoperoxide Synthases
Prostaglandins
RNA, Messenger
Triamcinolone
Adrenal Cortex Hormones
Budesonide
Cyclooxygenase 2
Dexamethasone
Indomethacin
Interleukin-1
Interleukin-1beta
Prostaglandin-Endoperoxide Synthases
Prostaglandins
RNA, Messenger
Triamcinolone