Korean J Otolaryngol-Head Neck Surg.
2000 Jul;43(7):746-750.
Loss of Heterozygosity on Chromosome 3p, 9p, 17p in Oral & Oropharyngeal Squamous Cell Carcinoma
- Affiliations
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- 1Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea. entkms@cmc.cuk.ac.kr
Abstract
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BACKGROUND AND OBJECTIVES: Patients of oral and oropharyngeal squamous cell carcinoma in the comparable stage have diverse clinical courses and responses to similar treatment. Understanding the genetic alterations that occur in these neoplasms may help us understan4 their biology and behavior. Loss of heterozygosity (I.OH) correlates with inactivated tumor suppressor genes, and tumor suppressor genes are important in the development of oral and oropharyngeal squamous cell carcinoma. We invested LOH for the p53, p16, FHlT gene loci by PCR using microsatellite markers. MATERIALS AND METHODS: Specimens from 25 patients with oral and oropharngeal sqamous cell carcinomas were microdissected, and DNA was obtained from normal and tumoral tissues from paraffin embedded sections. PCR analysis was done with three primers, TP53 (17p13.1) for p53 gene and D9S165 (9p21) for p16 gene and D3S1067 (3p21.1-3p14.2) for FHIT gene. LOH was defined as reduction in the allelic ratio over 50%. RESULTS: LOH at the p53 locus was found in 6/17 of the informative cases (35%), and for the p16 locus, LOH was found in 4/13 of the informative cases (31%). For FHIT locus, LOH was found in 4/15 of the informative cases (27%).
CONCLUSION
LOH on chromosome 3 (FHlT gene), chromosome 9 (p16 gene) and chromosome 17 (p53 gene) occurs in oral and oropharyngeal squamous cell carcinoma with high frequency suggesting that these tumor suppressor genes are involved in the development and progression of these carcinomas. There was no correlation behveen genetic alterations, age, pathologic stage, depth of invasion, cervical lymph node metastasis, recurrence and differentiation.
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