Korean J Obstet Gynecol.  2003 Apr;46(4):810-819.

cDNA Microarray Expression Analysis in HPV-Infected Uterine Cervical Cancer Cell Line

• Hur K
• Lin Z
• Zhang X
• Park CW
• Kim HO
• Cho JH
• Kang IS
• Kim YS
• Kim IS

Abstract

OBJECTIVE
To estimate the difference in gene expression related to carcinogenesis between HPV 16 positive squamous cell carcinoma and HPV 16 positive adenocarcinoma of cervix.
METHODS
We used cDNA microarray technology to identify alterations in gene expression of human cervical cancers. Gene expression of three cell lines, CaSki and SiHa (HPV 16 positive squamous cell carcinoma) and HeLa (HPV 16 positive adenocarcinoma) were compared with HT3 (HPV 16 negative squamous cell carcinoma). The microarray contains twin spots for 344 cancer-associated genes.
RESULTS
The analysis showed several interesting findings: (1) In all three squamous cell lines, CD4, CSF1, MMP15 and TNFR6 were increased, whereas SLC3A2 were decreased, (2) Only in adenocarcinoma cell line HeLa, CDC25A, CDK2, CDK9, IL2, PF4, MAD, FCER2, MAP4K1 and MS4A1 were increased, and PLAU, IL8, IL9R and ATK were decreased. (3) In both squamous cell carcinoma cell lines CaSki and SiHa, 61 genes which belong to chemokine, cell cycle, growth factor, interleukin, adhesion molecule, protein kinase and TNF were increased, whereas 10 genes which are associated with apoptosis and cytokine were increased only in SiHa, and 97 genes which are associated with a variety of cell functions were increased only in CaSki.
CONCLUSION
We suggest that there might be common, but also different carcinogenic mechanisms involved in HPV 16 related cervical cancers according to the histologic subtypes and different tumors.

Keyword

Cervical cancer cell line; cDNA microarray; Gene expression

MeSH Terms

Adenocarcinoma
Apoptosis
Carcinogenesis
Carcinoma, Squamous Cell
Cell Cycle
Cell Line*
Cervix Uteri
DNA, Complementary*
Female
Gene Expression
Human papillomavirus 16
Humans
Interleukin-2
Interleukin-8
Interleukins
Oligonucleotide Array Sequence Analysis*
Protein Kinases
Uterine Cervical Neoplasms*
DNA, Complementary
Interleukin-2
Interleukin-8
Interleukins
Protein Kinases