Gene Profiling of Uterine Cervical Carcinoma by cDNA Microarray

Yoo, Tae Sik; Park, Jong Tae; Nam, Jong Hee

Abstract

It is well known that human papillomavirus (HPV), especially the high-risk HPV types 16 and 18, is the one of the potent etiological agents in the development of cervical cancer. However, only a small fraction of the women with HPV develops cervical cancer, indicating that additional factors and molecular events are required for cervical carcinogenesis. In order to understand how the altered expression of a specific gene plays an integral role in cervical carcinogenesis, we examined the profiling of differentially expressed genes from cells of the normal squamous epithelium, carcinoma in situ (CIS) and invasive carcinoma of uterine cervix, using GeneFilter cDNA microarray. Hybridization of the GeneFilter cDNA membrane was performed with 33P-labeled cDNA probes synthesized from RNA amplified and isolated from about 20,000 cells of normal epithelium, CIS or invasive cervical carcinoma by laser capture microdissection. Parallel analyses of the hybridized signals enabled to profile genes that were differentially expressed in cervical carcinoma. In the carcinoma cells of CIS and invasive carcinoma, several genes including HOXB7, ESRRA, LAF4, CKS2, SC65, MCRS1, AKAP2, ENIGMA, PTK7, GCN5L1, DKFZP434, FLJ10422, C6ORF1, HLA-DQB1, and HSPA1B were detected in increased levels. The genes that showed increased expression in the cells of invasive carcinoma were ROCK1, CNTN1, RELN, CRTL1, DKFZP564B, CSNK2A2, GPRK5, 13CDNA73, and SMP-1. The genes that revealed decreased expression in cervical carcinoma cells were HNRPA1, TOP1, IFI16, DNMT1, SMCX, DDB2, DKFZP586B0519, AOE372, YDD19, FLJ20580, and SAT. These results indicated that the profiling of differentially expressed genes can be analysed by GeneFilter cDNA microarray in cervical carcinoma. The precise relationship between the altered expression of these genes and cervical tumorigenesis is a subject of further investigation.