Korean J Orthod.  2001 Apr;31(2):237-248.

Effects of estrogen and progesterone on the proliferation and activity of osteoblastic cells

• Ha KB
• Son WS
• Kim SW

Abstract

Biomechanical reactions of tooth movement are the combination of bone formation and resorption, in which many paracrine factors are involved. The sex hormone is one of the paracrine factors and the sex hormonal level of an adult female varies according to the body condition, e.g. mensturation, pregnancy, postmenopause, etc. Although the exact mechanism is not clarified yet, estrogen and progesterone are known to regulate the function of osteoblast. Again osteoblast is reported to affect the function of osteoclast. The purpose of this study is to determine the influence of the female sex hormone, estrogen and progesterone, on the cell proliferation and activity of HOS and ROS17/2.8 cell line. The observed results were as follows. 1. Estrogen inhibited HOS cell proliferation and promoted ROS17/2.8 cell proliferation. 2. Estrogen increased the activity of alkaline phosphatase of HOS cell and reduced the activity of alkaline phosphatase of ROS17/2.8 cell. 3. Progesterone inhibited the proliferation of HOS and ROS17/2.8 cell, but had no influence on the activity of alkaline phosphatase. 4. Estrogen and progeterone did not have any particular effects on the activity of super oxide, nitric oxide and gelatinase of HOS and ROS17/2.8 cell.

Keyword

Progesterone; Estrogen; Osteoblast; MTT; Alkaline phosphatase; Nitrite NBT gelatinase

MeSH Terms

Adult
Alkaline Phosphatase
Cell Line
Cell Proliferation
Estrogens*
Female
Gelatinases
Humans
Nitric Oxide
Osteoblasts*
Osteoclasts
Osteogenesis
Postmenopause
Pregnancy
Progesterone*
Tooth Movement
Alkaline Phosphatase
Estrogens
Gelatinases
Nitric Oxide
Progesterone