Korean J Obstet Gynecol.  2001 Apr;44(4):748-754.

Regulatory Mechanism of COX-2 by Estrogen and Progesterone in Uterine Cell Lines of Rodents

• Kang BM
• Park EJ
• Kim SH
• Cheon DJ
• Chae HD
• Kim CH
• Park K
• Chang YS

Abstract

OBJECTIVE
To evaluate the regulatory mechanism of cyclooxygenase(COX)-2 by estrogen and progesterone in uterine cell lines of rodents
MATERIALS AND METHODS
CUE-P cells, originated from the uterine endometrial epithelial cells, and CUE-V2 cells, originated from the uterine endometrial stromal cells, were incubated in the Dulbecco's modified Eagle's medium(DMEM) and F12 medium. The cellular proliferation were evaluated every 24 hours. The expression of COX-2 was measured after estrogen and progesterone supplementation by western blot.
RESULTS
CUE-P cells have cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. When CUE-P and CUS-V2 cells were co-cultured with estrogen and progesterone supplementation, the expression of COX-2 was decreased after 24 hours of estrogen supplementation. The decreased expression of COX-2 was observed only when estrogen was supplemented after 9 hours of initial progesterone supplementation.
CONCLUSION
The sex steroid, such as estrogen and progesterone could regulate COX-2 expression in uterine cell lines.

Keyword

cyclooxygenase-2; CUE-P; CUE-V2; estrogen; progesterone; expression

MeSH Terms

Blotting, Western
Cell Line*
Cell Proliferation
Cyclooxygenase 2
Epithelial Cells
Estrogens*
Fibroblasts
Progesterone*
Rodentia*
Stromal Cells
Cyclooxygenase 2
Estrogens
Progesterone