Korean J Dermatol.
2006 May;44(5):579-585.
Expression of Human Melanocyte Markers in PMA-containing Medium and Physiologic Mitogen-containing Medium
- Affiliations
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- 1Department of Dermatology, School of Medicine, Gyeongsang Institute of Health Science, Gyeongsang National University, Jinju, Korea. yoontj@gsnu.ac.kr
- 2Department of Dermatology, School of Medicine, Dong-A University, Korea.
- 3Immunologic Research, Kyunghee University, Seoul, Korea.
- 4Department of Dermatology, Kyunghee University, Seoul, Korea.
Abstract
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BACKGROUND: There are many growth media for cultivation of human melanocytes (MGM), depending on the supplements added, and the growth of cells is closely related to these components. To understand melanocytes in vivo, it is necessary to find out the biological or biochemical characteristics of melanocytes grown in physiologic growth medium (P-MGM) and phorbol-12-myristate 13-acetate (PMA)-containing medium (C-MGM).
OBJECTIVE
To investigate the expression of different biochemical markers of melanocytes grown in C-MGM and in P-MGM.
METHODS
C-MGM is basically composed of PMA (10 ng/ml), and bFGF (3 ng/ml), and is now commercially available for melanocyte culture. P-MGM is a physiologic growth medium containing physiologic mitogens such as bFGF (10 ng/ml), ET-1 (10 nM), and alpha-MSH (12 nM). The cell proliferation and the expression of biochemical markers were measured in cultured human melanocytes which were grown in either C-MGM or P-MGM.
RESULTS
In this study, there was significant difference in cell proliferation between cells grown in C-MGM and P-MGM (p<0.01). The tyrosinase activity and melanin contents were significantly increased in C-MGM. The expression of TRP1, MART-1 and p53 in mRNA level was higher in C-MGM than in P-MGM. The up-regulation of p53 protein expression was also observed in C-MGM.
CONCLUSION
The proliferation and expression of p53, at both transcriptional and translational levels were increased when melanocytes were grown in C-MGM, compared to P-MGM. This data suggests that p53-mediated melanization is to some degree related with phorbol ester, and should further be elucidated.
Keyword
- Melanocyte; MGM; Phorbol ester; PMA; p53
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