Korean J Androl.  2008 Sep;26(3):123-129.

Expression Changes of the Phosphodiesterase 5 isoform in the Penis of Type 2 Diabetic Rat

• Ko K
• Kim HJ
• Han YK
• Shim JB
• Kim SY
• Kim H
• Yang DY

Abstract

PURPOSE: To investigate the tissue distribution of PDE5 isoforms in type 2 diabetic rat penile tissues.
MATERIALS AND METHODS
We prepared ten male Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which develop NIDDM naturally, and ten control male Long-Evans Tokushima Fatty (LETO) rats. An oral glucose tolerance test confirmed diabetes development in OLETF rats at 26 weeks. At 42 weeks, we checked serum glucose, testosterone, triglyceride, insulin, and adiponectin before sacrifice. We performed semi-quantitative multiplex RT-PCR for rat PDE5, PDE5A1, and PDE5A2. Immunohistochemistry was performed using mouse monoclonal anti-cGB-PDE5 and anti-smooth muscle alpha-actin.
RESULTS
OLETF rats were significantly more hyperglycemic, hypogonadal, hyperinsulinemic, hypercholesterolemic, hypertriglycemic, and had lower adiponectin levels than LETO rats. Levels of PDE5 mRNA were decreased in OLETF rats, but there were no changes in PDE5A1 or PDE5A2 mRNA levels.
CONCLUSION
Diabetes may contribute to decreased expression of PDE5 mRNA, but not PDE5A1 or PDE5A2, in rat penile tissue. Furthermore, serum free testosterone was decreased in diabetic rats. PDE5 has an important role in the development of diabetic erectile dysfunction, but it is not clear whether PDE5A1 and PDE5A2 gene have specific roles.

Keyword

PDE5 protein; Diabetes Mellitus; Penis; Rat

MeSH Terms

Actins
Adiponectin
Animals
Cyclic Nucleotide Phosphodiesterases, Type 5
Diabetes Mellitus
Diabetes Mellitus, Type 2
Erectile Dysfunction
Glucose
Glucose Tolerance Test
Humans
Immunohistochemistry
Insulin
Male
Mice
Muscles
Penis
Protein Isoforms
Rats
Rats, Inbred OLETF
RNA, Messenger
Testosterone
Tissue Distribution
Actins
Adiponectin
Cyclic Nucleotide Phosphodiesterases, Type 5
Glucose
Insulin
Protein Isoforms
RNA, Messenger
Testosterone