J Korean Ophthalmol Soc.
2003 Dec;44(12):2890-2895.
Oxidative Damage induced by UV in Cultured Retinal Pigment Epithelial Cells
- Affiliations
-
- 1Department of Ophthalmology, Seoul National University, College of Medicine, Seoul Artificial Eye center, Seoul National University Hospital, Clinical Research Institute, Korea. chungh@snu.ac.kr
Abstract
- PURPOSE
The death of retinal pigment epithelial(RPE) cells plays an important role in the development of age-related macular degeneration. We studied whether ultraviolet could induce apoptotic cell death and the increase of reactive oxygen intermediates (ROI) in the RPE cells. METHODS: Cultured human RPE cells were exposed to doses of between 0 and 80 mJ/cm2 UVB as an attached monolayer. The number of viable cells was assessed by trypan blue assay. The apoptosis was determined by flow cytometry using annexin V and propidium iodide (PI). Intracellular ROI was measured using DCFH-DA. RESULTS: UVB-exposed cells showed a dose-dependent cell death. The number of cells decreased in 20~30% at the dose of 40 mJ/cm2 UVB 24 hours after UVB exposure. The proportion of annexin V positive cells and intracellular ROI increased after UVB exposure. CONCLUSIONS: These results suggest that UV-induced oxidative damage may be one of the important mechanisms of RPE cell death.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Protective Effects of Epigallocatechin Gallate after UV Irradiation in Cultured Human Retinal Pigment Epithelial Cells
- The Effect of 5-Fluorouraci1 on the Activity of the Retinal Pigment Epithelium in Vitro
- Culture of Retinal Pigment Epithelial Cells on Collagen Membrane
- Growth Patterns of Human Retinal Pigment Epithelium in Vitro
- Histologic Study in Transplantation of Cultured Rabbit Retinal Pigment Epithelium
