J Korean Soc Coloproctol.  2010 Aug;26(4):254-259. 10.3393/jksc.2010.26.4.254.

Effects of DCA on Cell Cycle Proteins in Colonocytes

Affiliations
  • 1Department of Surgery, Dankook University School of Medicine, Cheonan, Korea. dkpark@dankook.ac.kr

Abstract

PURPOSE
Evidence that indicates bile acid is a promoter of colon cancer exists. Deoxycholic acid (DCA) modifies apoptosis or proliferation by affecting intracellular signaling and gene expression. However, because previous studies have been based on studies on colon cancer cell lines, the effect of DCA on normal colonocytes is unknown.
METHODS
Normal colonocytes and Caco-2 and HCT116 cells were treated with 20 micrometer and 250 micrometer of DCA, and the effect of different concentrations of DCA was measured based on the expression of cell-cycle-related proteins by using Western blots.
RESULTS
The expressions of CDK2 and cyclin D1 for different concentrations of DCA in normal colonocytes and colon cancer cells were similar, but the expressions of cyclin E and A were significantly different. In HCT116 colon cancer cells, the expression of cyclin E increased regardless of the DCA concentration, but in normal colonocytes and Caco-2 cells, the expression of cyclin E was not changed or decreased. In HCT116 colon cancer cells, the expression of cyclin A was not changed or decreased regardless of the DCA concentration, but in normal colonocytes and Caco-2 cells, the expression of cyclin A was increased at a DCA concentration of 20 micrometer.
CONCLUSION
The effect of DCA on stimulating cell proliferation suggests that DNA synthesis is stimulated by an increased expression of cyclin E in colon cancer cells. Our results suggest that a low dose of DCA induces cellular proliferation through increased expression of cyclin A and that a high dose of DCA induces decreased expression of cyclin E and CDK2 in normal colonocytes.

Keyword

Deoxycholic acid; Cell cycle; Cyclin; Colonocyte; CDK2

MeSH Terms

Apoptosis
Bile
Blotting, Western
Caco-2 Cells
Cell Cycle
Cell Cycle Proteins
Cell Line
Cell Proliferation
Colonic Neoplasms
Cyclin A
Cyclin D1
Cyclin E
Cyclins
Deoxycholic Acid
DNA
Gene Expression
HCT116 Cells
Humans
Proteins
Cell Cycle Proteins
Cyclin A
Cyclin D1
Cyclin E
Cyclins
DNA
Deoxycholic Acid
Proteins
Full Text Links
  • JKSC
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr