J Korean Assoc Maxillofac Plast Reconstr Surg.  2005 Jan;27(1):16-23.

The effect of rhBMP-2 in human bone marrow-derived stem cells as osteogenic inducers

• Kim IS
• Zhang YL
• Cho TH
• Lee KB
• Park YD
• Rho IS
• Weber F
• Hwang SJ
• Kim MJ
• Lee JH

Abstract

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

Keyword

BMP-2; Bone marrow-derived stem cell(BMSC); Dexamethasone(Dex); Osteogenesis; ALP(Alkaline phosphatase)

MeSH Terms

Alkaline Phosphatase
Animals
Bone Marrow
Child
Collagen
Dexamethasone
Humans*
Integrin-Binding Sialoprotein
Mice
Osteoblasts
Osteocalcin
Osteogenesis
Osteopontin
Rats
Rodentia
Stem Cells*
Stromal Cells
Tissue Donors
Vitamin D
Alkaline Phosphatase
Collagen
Dexamethasone
Integrin-Binding Sialoprotein
Osteocalcin
Osteopontin
Vitamin D