Intest Res.  2014 Jul;12(3):205-213. 10.5217/ir.2014.12.3.205.

Expression of Heat Shock Proteins and Cytokines in Response to Ethanol Induced Damage in the Small Intestine of ICR Mice

Affiliations
  • 1Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Korea. schlp@hanmail.net
  • 2Department of Pharmacy, Kangwon National University School of Medicine, Chuncheon, Korea.
  • 3Department of Pathology, Kangwon National University School of Medicine, Chuncheon, Korea.

Abstract

BACKGROUND/AIMS
Ethanol administration causes intestinal epithelial cell damage by increasing intestinal permeability and the translocation of endotoxins from intestinal bacterial flora. Heat shock proteins (HSPs) are associated with recovery and protection from cell damage. The aim of the current study was to investigate differences in the expression of HSPs in the small intestine and the biochemical changes attributable to ethanol-induced intestinal damage.
METHODS
Ethanol (20%) was injected intraperitoneally (2.75 g/kg, 5.5 g/kg, 8.25 g/kg) in ICR mice and the same volume of saline was administered to controls. After 1 hour, the proximal, middle, and distal segments were taken from the small intestine and the degree of damage was analyzed. In each segment, the expression of HSPs was analyzed by western blotting. The expression of inflammatory mediators including interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), cyclooxygenase-2 (COX-2), and antioxidant enzyme such as glutathione-S-transferase were compared using real-time polymerase chain reaction assays.
RESULTS
In the control group, HSP70 increased in all segments of small intestine. Additionally, increases in the expression of HSP40 and HSP90 in the distal regions and an increase in HSP32 in the middle regions were observed. After ethanol treatment, greater histological damage was observed in the distal small intestine and significant decreases in HSPs were observed generally. Increased expression of IL-1beta, TNF-alpha, and COX-2 was observed in small intestinal tissues exposed to ethanol-induced damage. However, there was no significant difference in the expression of an antioxidant enzyme.
CONCLUSIONS
Significant differences in the expression of HSPs in different intestinal regions were observed. These differences may have been attributable to the distribution of intestinal bacteria.

Keyword

Intestine, small; Heat shock protein; Ethanol

MeSH Terms

Animals
Bacteria
Blotting, Western
Cyclooxygenase 2
Cytokines*
Endotoxins
Epithelial Cells
Ethanol*
Heat-Shock Proteins*
Interleukin-1beta
Intestine, Small*
Mice
Mice, Inbred ICR*
Permeability
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Cyclooxygenase 2
Cytokines
Endotoxins
Ethanol
Heat-Shock Proteins
Interleukin-1beta
Tumor Necrosis Factor-alpha
Full Text Links
  • IR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr