J Korean Acad Rehabil Med.  2009 Feb;33(1):1-4.

The Effect of Steroid on Heat Shock Protein 70 Expression in mdx Mice

Affiliations
  • 1Department of Rehabilitation Medicine, Konkuk University Medical Center, Korea. jhlim@kuh.ac.kr
  • 2Department of Rehabilitation Medicine, Seoul National University College of Medicine, Korea.

Abstract


OBJECTIVE
To investigate the effect of steroid administration on the apoptosis and heat shock protein 70 (HSP70) expression after exercise in the animal model of Duchenne muscular dystrophy. METHOD: We measured Bcl-2, BAX and HSP70 expression by western blotting. 20 control and 20 mdx mice were divided into free-living (n=10) and exercise (n=10) groups. Free-living and exercise groups were further divided into steroid-treated and sham-treated groups to evaluate the effect of steroid administration.
RESULTS
Apoptosis was most prominent in the sham-treated exercise group, while apoptosis was significantly reduced in the steroid-treated exercise group. HSP70 expression was maximized in sham-treated exercise group, whereas steroid administration inhibited HSP70 expression after exercise in muscular dystrophy animal model. Exercise loading was found to cause severe apoptosis but steroid administration alleviated apoptotic damage in mdx mice.
CONCLUSION
HSP70 expression was suppressed in the steroid-treated exercise group, which suggests steroid might have major preventive effect in exercise-induced apoptosis of muscular dystrophy animal model.

Keyword

mdx mouse; Steroid; Exercise; Heat shock protein

MeSH Terms

Animals
Apoptosis
Blotting, Western
Heat-Shock Proteins
Hot Temperature
HSP70 Heat-Shock Proteins
Mice
Mice, Inbred mdx
Models, Animal
Muscular Dystrophies
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
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