J Korean Burn Soc.  2012 Dec;15(2):96-101.

The Therapeutic Effect of Porcine Placenta Extract for Improvement Sequelae of Burn

• Kim HS
• Lee JH
• Yeon CJ
• Lee JS

Abstract

PURPOSE
Burn injury cause pruritis, pain, psychological and functional sequelae. The one of burn injury sequelae is the hypertrophic scar. It is difficult to control devastating fibrotic condition for hypertrophic scar. The objective of this study was to investigated the therapeutic effect on burn hypertrophic scar and wound healing for sequelae of burn injury by Porcine placenta extract (PPE).
METHODS
To investigate the effect of PPE, we performed in vitro cell cytotoxity test (MTT assay), antioxidant activity assay (SOD like activity), melanin content assay, cell migration asssay and RT-PCR.
RESULTS
As a result of cell cytotoxity test (MTT assay), PPE showed above 80% cell viability. From Antioxidant activity assay (SOD like activity), this effect was similar to vitamin C. In the melanin content assay, melanin synthesis was inhibited 23% on PPE treatment than control. PPE enhanced cell migration on human fibroblast and decreased the expression of hypertropic scar related gene (a-SMA and P311).
CONCLUSION
Our data showed anti-oxidant effect, diminution of melanin and decrease of the expression of hypertropic scar related gene on the treatment of PPE. These results may provide the insight into the potential use of porcine placenta extract as support to control skin fibrosis related to burn hypertrophic scar and alternative medicine for burn sequelae.

Keyword

Porcine placenta extract (PPE); Wound healing; Melanin synthesis inhibition; SOD like activity; Hypertropic scar

MeSH Terms

Antioxidants
Ascorbic Acid
Burns
Cell Migration Assays
Cell Movement
Cell Survival
Cicatrix
Cicatrix, Hypertrophic
Complementary Therapies
Fibroblasts
Fibrosis
Humans
Melanins
Placenta
Pruritus
Skin
Wound Healing
Antioxidants
Ascorbic Acid
Melanins