J Cancer Prev.  2015 Mar;20(1):50-56. 10.15430/JCP.2015.20.1.50.

Anthocyanins From the Fruit of Vitis coignetiae Pulliat Potentiate the Cisplatin Activity by Inhibiting PI3K/Akt Signaling Pathways in Human Gastric Cancer Cells

Affiliations
  • 1Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju, Korea. lwshmo@gnu.ac.kr
  • 2Department of Surgery, Konkuk University School of Medicine, Seoul, Korea.
  • 3Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan, Korea.
  • 4Department of Pharmacology, Gyeongsang National University School of Medicine, Korea.
  • 5School of Veterinary Medicine, Institute of Agriculture and Life Science, Korea.
  • 6Division of Applied Life Science (BK 21 Program), Institute of Agriculture and Life Science, Korea.
  • 7Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Korea.
  • 8Department of Neurosurgery, Gyeongsang National University School of Medicine, Jinju, Korea.
  • 9Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.

Abstract

BACKGROUND
Cisplatin (cis-diaminedichloroplatinum, CDDP) is a widely used chemotherapeutic agent for the treatment of many cancers. However, initial resistance to CDDP is a serious problem in treating these cancers. Vitis coignetiae Pulliat (Meoru in Korea) have shown anti-nuclear factor kappa B and anti-epidermal growth factor receptor activities in cancer cells.
METHODS
In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products. Here, we investigated anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) can enhance anti-cancer effects of cisplatin (CDDP) in stomach cancer cells. The cell viability of SNU-1 and SNU-16 cells after treated with AIMs and CDDP were analyzed by MTT assay. The expressions of Akt and X-linked inhibitor of apoptosis protein (XIAP) proteins were examined by western blot in AIMs- and CDDP-treated cells.
RESULTS
We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic. AIMs suppressed Akt activity of the cancer cells activated by CDDP. AIMs also suppressed in XIAP an anti-apoptotic protein.
CONCLUSIONS
This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.

Keyword

Anthocyanins; Cisplatin; Stomach neoplasms

MeSH Terms

Anthocyanins*
Biological Products
Blotting, Western
Cell Survival
Cisplatin*
Fruit*
Humans*
Stomach Neoplasms*
Vitis*
X-Linked Inhibitor of Apoptosis Protein
Anthocyanins
Biological Products
Cisplatin
X-Linked Inhibitor of Apoptosis Protein
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