Biomol Ther.  2016 Jan;24(1):62-66. 10.4062/biomolther.2015.172.

Amygdalin Regulates Apoptosis and Adhesion in Hs578T Triple-Negative Breast Cancer Cells

• Lee HM
• Moon A

• KMID: 2170542
• DOI: http://doi.org/10.4062/biomolther.2015.172

Abstract

Amygdalin, D-mandelonitrile-beta-D-glucoside-6-beta-glucoside, belongs to aromatic cyanogenic glycoside group derived from rosaceous plant seed. Mounting evidence has supported the anti-cancer effects of amygdalin. However, whether amygdalin indeed acts as an anti-tumor agent against breast cancer cells is not clear. The present study aimed to investigate the effect of amygdalin on the proliferation of human breast cancer cells. Here, we show that amygdalin exerted cytotoxic activities on estrogen receptors (ER)-positive MCF7 cells, and MDA-MB-231 and Hs578T triple-negative breast cancer (TNBC) cells. Amygdalin induced apoptosis of Hs578T TNBC cells. Amygdalin downregulated B-cell lymphoma 2 (Bcl-2), upregulated Bcl-2-associated X protein (Bax), activated of caspase-3 and cleaved poly ADP-ribose polymerase (PARP). Amygdalin activated a pro-apoptotic signaling molecule p38 mitogen-activated protein kinases (p38 MAPK) in Hs578T cells. Treatment of amygdalin significantly inhibited the adhesion of Hs578T cells, in which integrin alpha5 may be involved. Taken together, this study demonstrates that amygdalin induces apoptosis and inhibits adhesion of breast cancer cells. The results suggest a potential application of amygdalin as a chemopreventive agent to prevent or alleviate progression of breast cancer, especially TNBC.

Keyword

Amygdalin; Apoptosis; Adhesion; Breast cancer

MeSH Terms

Adenosine Diphosphate Ribose
Amygdalin*
Apoptosis*
bcl-2-Associated X Protein
Breast Neoplasms
Caspase 3
Humans
Integrin alpha5
Lymphoma, B-Cell
MCF-7 Cells
p38 Mitogen-Activated Protein Kinases
Plants
Receptors, Estrogen
Triple Negative Breast Neoplasms*
Adenosine Diphosphate Ribose
Amygdalin
Caspase 3
Integrin alpha5
Receptors, Estrogen
bcl-2-Associated X Protein
p38 Mitogen-Activated Protein Kinases