J Biomed Res.  2014 Dec;15(4):182-188. 10.12729/jbr.2014.15.4.182.

Characteristics and immuno-modulatory effects of Enterococcus faecium JS1-8 isolated from Kimchi

• Ahn SB
• Park HE
• Lee SM
• Kim SY
• Lee WK

• KMID: 2168997
• DOI: http://doi.org/10.12729/jbr.2014.15.4.182

Abstract

Characteristics and immuno-modulatory effects of Enterococcus (E.) faecium JS1-8 isolated from Kimchi were investigated for potential probiotic use. We measured their acid, bile, and heat tolerances, adhesion properties in Caco-2 cells, antimicrobial activity against pathogenic bacteria, and bacteriocin-like substance-producing activity. Immuno-modulatory effects of E. faecium JS1-8 were measured by determination of nitric oxide (NO), nuclear factor-kappaB (NF-kappaB), interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) production in RAW 264.7 cells or RAW BLUE cells. JS1-8 survived at pH 2.0 for 2 hr and showed tolerance to 0.3% oxgall bile salt, and it survived after exposure for 5 min at 80degreesC. JS1-8 showed high antimicrobial inhibition zones to Staphylococcus aureus (460 mm), Listeria monocytogenes (310 mm), Salmonella enteritidis (280 mm), and E. coli (150 mm). Bacteriocin-like substance produced by JS1-8 showed a broad spectrum of activity against Listeria monocytogenes KCCM 40307 and Lactobacillus sake KCCM 40264. Low concentration (1 x 107 CFU/mL) of heat-killed E. faecium JS1-8 induced statistically higher production of NO than Lactobacillus rhamnosus GG (LGG), which is a well-known immuno-modulatory lactic acid bacteria. Low and high (5 x 107 CFU/mL) concentrations of JS1-8 induced statistically higher production of NF-kappaB than that produced by LGG. We also found that JS1-8 increased production of pro-inflammatory cytokines such as IL-1beta and TNF-alpha in a concentration-dependent manner. As a result, E. faecium JSI-8 could be used as a useful probiotic for controlling pathogens and enhancing host immune responses.

Keyword

Enterococcus faecium; bacteriocin; probiotics; immuno-modulatory effect; Kimchi

MeSH Terms

Bacteria
Bile
Caco-2 Cells
Cytokines
Enterococcus
Enterococcus faecium*
Hot Temperature
Humans
Hydrogen-Ion Concentration
Interleukin-1beta
Lactic Acid
Lactobacillus
Lactobacillus rhamnosus
Listeria monocytogenes
NF-kappa B
Nitric Oxide
Probiotics
Salmonella enteritidis
Staphylococcus aureus
Tumor Necrosis Factor-alpha
Cytokines
Interleukin-1beta
Lactic Acid
NF-kappa B
Nitric Oxide
Tumor Necrosis Factor-alpha