J Korean Acad Periodontol.  2008 Mar;38(1):31-40. 10.5051/jkape.2008.38.1.31.

Inhibitory effects of Enterococcus faecium isolated from Korean infants on oral pathogens

• Jeong EG
• Lee JC
• Seo JY
• Kim SY
• Kim WS
• Yun WH
• Kim YS
• Pi SH
• You HK
• Shin HS

• KMID: 1486782
• DOI: http://doi.org/10.5051/jkape.2008.38.1.31

Abstract

PURPOSE
The probiotic effects of lactic acid bacteria have widely been researched in diverse human pathogens, but only a few effects are reported against oral pathogens. The antimicrobial effects of the Enterococcus faecium 7413 isolated from Korean infants on the 9 pathogen including 6 oral streptococci were investigated the clinical use of the antimicrobial peptide for oral microflora control.
MATERIALS AND METHODS
E. faecium 7413 was identified by morphological, biochemical tests and 16S rDNA sequence analysis. Inhibitory effects of culture supernatants were determined for their ability to grow on agar plate containing pathogenic bacteria. RESULT: The culture supernatant of Enterococcus faecium 7413 showed inhibitory effects on oral pathogens, namely Streptococcus pyogenes KCTC 3556, S. pneumoniae KCTC 5080, S. mutans ATCC 25175, S. anginosus ATCC 33397, S. constellatus KCTC 3268, S. intermedius ATCC 27823 and Shigella flexneri KCTC 2008. Whereas it did not affect the multiplication of E. coli strains, KCTC 1041 and ATCC 43894.
CONCLUSION
The data obtained in this study could be useful for future development of effective probiotics allowing prevention for oral pathogens.

Keyword

Enterococcus faecium; infant; oral pathogens

MeSH Terms

Agar
Bacteria
DNA, Ribosomal
Enterococcus
Enterococcus faecium
Humans
Infant
Lactic Acid
Pneumonia
Probiotics
Sequence Analysis
Shigella flexneri
Streptococcus pyogenes
Agar
DNA, Ribosomal
Lactic Acid