J Biomed Res.  2013 Dec;14(4):206-211.

Characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, Korea traditional fermented food, for probiotic use

• Ahn SB
• Park HE
• Lee SM
• Kim SY
• Shon MY
• Lee WK

Abstract

In this study, characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, traditional Korean fermented food, were examined for investigation of the capacity of potentially probiotic strains. We measured acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. JW15 could survive at pH 3.0 for 2 hr, but not at pH 2.0. JW15 also showed tolerance to 0.3% oxgall bile salt, and heat tolerance at 70degrees C and 80degrees C for 5 min, respectively. Adhesive ability to Caco-2 cells was similar to that of Lactobacillus rhamnosus GG (LGG), a well-known commercial probiotic. JW15 exhibited antimicrobial activities to pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella enteritidis. The immuno-modulatory effects of JW15 were compared with those of LGG, a well-known immune enhancer. For analysis, production of nitric oxide (NO), NF-kappaB (Nuclear factor kappaB), Interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) was measured. The concentration of NO induced by JW15 was higher than that by LGG at low concentration (1 x 10(7) cfu/mL). Low and high (5 x 10(7) CFU/mL) concentration of JW15 induced statistically higher production of NF-kappaB, IL-1beta, and TNF-alpha than that produced by LGG, respectively. In conclusion, Weissella cibaria JW15 had ability as a probiotic strain, including acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. In addition, JW15 showed better immuno-modulatory effects than LGG when NO, NF-kappaB, IL-1beta, and TNF-alpha were measured. According to these results, the characteristics and immunomodulating activity of Weissella cibaria JW15 are suitable for consideration as a potential probiotic.

Keyword

Weissella cibaria; JW15; probiotics; immuno-modulatory effect; Kimchi

MeSH Terms

Adhesives
Bacteria
Bile
Caco-2 Cells
Epithelial Cells
Escherichia coli
Hot Temperature
Humans
Hydrogen-Ion Concentration
Interleukin-1beta
Korea*
Lactobacillus rhamnosus
Listeria monocytogenes
NF-kappa B
Nitric Oxide
Probiotics*
Salmonella enteritidis
Staphylococcus aureus
Tumor Necrosis Factor-alpha
Weissella*
Adhesives
Interleukin-1beta
NF-kappa B
Nitric Oxide
Tumor Necrosis Factor-alpha