J Bacteriol Virol.  2011 Dec;41(4):279-286. 10.4167/jbv.2011.41.4.279.

Isolation and Antimicrobial Susceptibility of Mupirocin-Resistant and Methicillin-Resistant Staphylococcus aureus from Clinical Samples

• Kim SM
• Park SY
• Park SD

• KMID: 2168655
• DOI: http://doi.org/10.4167/jbv.2011.41.4.279

Abstract

Resistance to mupirocin in methicillin-resistant Staphylococcus aureus (MRSA) have increased with wide use of mupirocin in many countries, but the prevalence in Korea is not well-known. The aim of this study was to determine the prevalence, antimicrobial susceptibility, and clonality of mupirocin-resistant (MUP-R) isolates from three Korean hospitals. A total of 175 MRSA isolates were collected from three university hospitals in 2009-2010. Antimicrobial susceptibility was tested by the disk diffusion and the agar dilution methods. femA, mecA and mupA genes were detected by polymerase chain reactions. Pulsed-filed gel electrophoresis (PFGE) pattern of genomic DNA was determined after digestion with SmaI. Overall, 12 among the 175 MRSA isolates were resistant to mupirocin, with prevalence ranging from 0 to 10% depending on hospitals. Three high-level (HL) and nine low-level (LL) MUR-R isolates were obtained from two hospitals. All MUP-R isolates were susceptible to rifampin and vancomycin, but were resistant to ciprofloxacin, clindamycin, and erythromycin. Eight LL and one HL MUP-R isolates were also resistant to fusidic acid. PFGE analysis showed three HL MUP-R isolates belonged to arbitrary cluster 3, 5 and 6 with 60~90% similarity compared to LL MUP-R isolates. In conclusion, the HL resistance to mupirocin was detected in two hospitals, but HL MUP-R isolates were clonally not related.

Keyword

Antimicrobial susceptibility; Mupirocin-resistant MRSA; mupA gene

MeSH Terms

Adenosine
Agar
Ciprofloxacin
Clindamycin
Diffusion
Digestion
DNA
Electrophoresis
Erythromycin
Fusidic Acid
Hospitals, University
Korea
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Mupirocin
Polymerase Chain Reaction
Prevalence
Rifampin
Vancomycin
Adenosine
Agar
Ciprofloxacin
Clindamycin
DNA
Erythromycin
Fusidic Acid
Mupirocin
Rifampin
Vancomycin

Figure

• 

  Figure 1. A dendrogram generated with Fingerprinting II informatix software showing the PFGE types (1–6) of SmaI-restricted chromosome DNA of 12 mupirocin-resistant MRSA isolates. No. 03, 07, and 08 isolates with high-level resistance to mupirocin; No. 01∼02, 4∼6, 9∼12 isolates with low-level resistance to mupirocin.

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