J Bacteriol Virol.  2009 Dec;39(4):307-316. 10.4167/jbv.2009.39.4.307.

Genotype, Coagulase Type and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Isolated from Dermatology Patients and Healthy Individuals in Korea

• Kim SM
• Lee DC
• Park SD
• Kim BS
• Kim JK
• Choi MR
• Park SY
• Hwang SM
• Shin NY
• Shim ES
• Kwon PS
• Kwon DY
• Hur SH
• Kim HJ
• Lim HB
• Chong Y

• KMID: 2168542
• DOI: http://doi.org/10.4167/jbv.2009.39.4.307

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most prevalent dermatology pathogens in hospitals and increasingly recognized in communities. We determined PFGE pattern of SmaI-restricted genomic DNA, coagulase type, and antimicrobial susceptibility of MRSA isolated in 2008 from dermatology inpatients and healthy hospital employees in A Hospital and from primary school children in Iksan city, Korea. Overall, the isolation rate of MRSA was 3.8% from the 788 normal persons: 4.9% from hospital employees and 1.1% from primary school children. MRSA was isolated in six of 13 (46.2%) family members of four school children with MRSA. The most prevalent coagulase serotype was II from patients and V from healthy individuals. Ten of twenty and six of twenty MRSA isolates from patients and from healthy personnel, respectively, had identical PFGE patterns, suggesting that these are originated from identical clones. Against MRSA from patients, only vancomycin was the most active (MIC range < or =2 microg/ml), whereas the resistance rates were 35% to rifampin and 65% to mupirocin. The resistance rates of patient isolates were > or =90% to amikacin, clindamycin, ciprofloxacin, erythromycin, fusidic acid, gentamicin and tetracycline. In conclusion, the MRSA carriage rates of healthy hospital workers were relatively high, 2.3~7.7%, depending on groups. Family members of a few primary school children with MRSA showed a high carriage rate, suggesting that intrafamily transmission occurred. MRSAs isolated from dermatology inpatients were relatively more resistant to various antimicrobial agents, including mupirocin, but all isolates were susceptibility to vancomycin.

Keyword

Genotype; Coagulase type; MRSA; Antimicrobial susceptibility

MeSH Terms

Amikacin
Anti-Infective Agents
Child
Ciprofloxacin
Clindamycin
Clone Cells
Coagulase
Dermatology
DNA
Erythromycin
Fusidic Acid
Genotype
Gentamicins
Humans
Inpatients
Korea
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Mupirocin
Rifampin
Tetracycline
Vancomycin
Natural Resources
Amikacin
Anti-Infective Agents
Ciprofloxacin
Clindamycin
Coagulase
DNA
Erythromycin
Fusidic Acid
Gentamicins
Mupirocin
Rifampin
Tetracycline
Vancomycin

Figure

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  Figure 1. PFGE dendrogram of SmaI-restricted DNA of MRSA isolates from dermatology patients and healthy persons. PFGE banding patterns were analyzed using the Dice coefficient and the unweighted pair group method with arithmetic averages (UPGMA). The coagulase type and presence of mecA gene are also shown.

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