J Bacteriol Virol.  2008 Mar;38(1):39-46. 10.4167/jbv.2008.38.1.39.

Human Cytomegalovirus Induces Intercellular Adhesion Molecule-1 Expression in a Monocytic Cell Line, THP-1

• Kim MS
• Yi HA
• Lee CH

• KMID: 2168517
• DOI: http://doi.org/10.4167/jbv.2008.38.1.39

Abstract

It has been reported that inflammatory diseases such as pneumonitis, retinitis, and hepatitis are associated with human cytomegalovirus (HCMV). Intercellular adhesion molecule (ICAM)-1 is an important inflammatory mediator, helping monocytes adhere to endothelial cells when tissues are infected by pathogen including the HCMV. However, little is known about the mechanism of ICAM-1 stimulation by the HCMV infection in monocytes. In this study, a monocytic cell line THP-1 was used to understand ICAM-1 expression by the HCMV infection. Flow cytometric analyses demonstrated that ICAM-1 was stimulated by the HCMV in THP-1 cells with maximum at 24 hours post infection. The stimulated ICAM-1 expression was dependent on the amount of input virus. In order to understand the mechanism of ICAM-1 stimulation during the HCMV infection, cells were treated with specific inhibitors of key elements in inflammation: NF-kappaB inhibitor PDTC, cyclooxygenase 2 inhibitor NS398, and MEK inhibitor PD98059. Flow cytometric analyses revealed that ICAM-1 expression was decreased when treated with PDTC, but not with NS398 or PD98059. Thus, it is suggested that HCMV-induced ICAM-1 expression in THP-1 cells is associates with NF-kappaB.

Keyword

HCMV; Inflammation; ICAM-1; NF-kappaB

MeSH Terms

Cell Line
Cyclooxygenase 2
Cytomegalovirus
Endothelial Cells
Flavonoids
Hepatitis
Humans
Inflammation
Intercellular Adhesion Molecule-1
Monocytes
NF-kappa B
Nitrobenzenes
Pneumonia
Proline
Retinitis
Sulfonamides
Thiocarbamates
Viruses
Cyclooxygenase 2
Flavonoids
Intercellular Adhesion Molecule-1
NF-kappa B
Nitrobenzenes
Proline
Sulfonamides
Thiocarbamates