J Gastric Cancer.  2012 Sep;12(3):140-148.

Expression of Intercellular Adhesion Molecule-1 and E-Selectin in Gastric Cancer and Their Clinical Significance

• Jung WC
• Jang YJ
• Kim JH
• Park SS
• Park SH
• Kim SJ
• Mok YJ
• Kim CS

Abstract

PURPOSE
Among cell adhesion molecules, serum levels of intercellular adhesion molecule-1 and E-selectin are known to be correlated with the metastatic potential of gastric cancer. In the present study, the authors investigated the expression of intercellular adhesion molecule-1 and E-selectin in gastric cancer tissues and cultured gastric cancer cells, and examined their clinical value in gastric cancer.
MATERIALS AND METHODS
The protein was extracted from gastric cancer tissues and cultured gastric cancer cells (MKN-28 and Kato-III) and the expression of intercellular adhesion molecule-1 and E-selectin was examined by western blotting. The clinical significance of intercellular adhesion molecule-1 and E-selectin was explored, using immunohistochemical staining of specimens from 157 gastric cancer patients.
RESULTS
In western blot analysis, the expressions of intercellular adhesion molecule-1 in gastric cancer tissues and cultured gastric cancer cells were increased, however, E-selectin in gastric cancer tissues and cells were not increased. Among 157 gastric cancer patients, 79 patients (50%) were intercellular adhesion molecule-1 positive and had larger tumor size, an increased depth of tumor invasion, lymph node metastasis and perineural invasion. The intercellular adhesion molecule-1 positive group showed a higher incidence of tumor recurrence (40.5%), and a poorer 3-year survival than the negative group (54.9 vs. 85.9%, respectively).
CONCLUSIONS
Intercellular adhesion molecule-1 is overexpressed in gastric cancer tissues and cultured gastric cancer cells, whereas E-selectin is not overexpressed. Increased expression of intercellular adhesion molecule-1 in gastric cancer could be related to the aggressive nature of the tumor, and has a poor prognostic effect on gastric cancer.

Keyword

Stomach neoplasms; Intercellular adhesion molecule-1; E-selectin

MeSH Terms

Blotting, Western
Cell Adhesion Molecules
E-Selectin
Humans
Incidence
Intercellular Adhesion Molecule-1
Lymph Nodes
Neoplasm Metastasis
Recurrence
Stomach Neoplasms
Cell Adhesion Molecules
E-Selectin
Intercellular Adhesion Molecule-1

Figure

• 

  Fig. 1 Intercellular adhesion molecule-1 (ICAM-1), E-selectin expression level in gastric cancer tissues and cell lines (MKN28, KATO3). (A) CAM-1 expression was noted in normal stomach tissues but it was increased in gastric cancer tissues and cultured gastric cancer cells. (B) E-selectin expression was more prominent in normal stomach tissues but it was decreased in gastric cancer tissues and cultured gastric cancer cells.

• 

  Fig. 2 Immunohistochemical staining of intercellular adhesion molecule-1 (ICAM-1), E-selectin in tissue microarray slides of gastric cancer. (A) Expression of ICAM-1 was noted in the endothelial cells of gastric cancer tissues. More than 50% of cancer cells were stained (×400). (B) Expression of E-selectin was noted in the diffuse type signet ring gastric cancer cells. More than 50% were stained (×400).

• 

  Fig. 3 (A) Overall 3-year survival of gastric cancer patients according to the intercellular adhesion molecule-1 (ICAM-1) immune reactivity. (B) Disease-free 3-year survival of gastric cancer patients according to the ICAM-1 immune reactivity.

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