Gut Liver.
2010 Dec;4(4):488-497.
Near-Infrared Fluorescence Imaging Using a Protease-Specific Probe for the Detection of Colon Tumors
- Affiliations
-
- 1Department of Internal Medicine, Chungbuk National University College of Medicine, Cheongju, Korea.
- 2Department of Internal Medicine and Asan Digestive Disease Research Institute, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. sjmyung@amc.seoul.kr
- 3Molecular Imaging Center, Asan Institute for Life Sciences, Seoul, Korea.
- 4Biomedical Research Center, Korea Institute of Science and Technology, Seoul, Korea.
- 5Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
- 6Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Abstract
- BACKGROUND/AIMS
Early tumor detection is crucial for the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging using a target-activatable probe may permit earlier disease detection. Matrix metalloproteinases (MMPs) participate in tumorigenesis and tumor growth. The aim of this study was to determine whether NIRF imaging using an MMP-activatable probe can detect colon tumors at early stages.
METHODS
We utilized two murine colon cancer models: a sporadic colon cancer model induced by azoxymethane (AOM), and a colitis-associated cancer model induced by a combination of AOM and dextran sodium sulfate (DSS). Colonic lesions were analyzed by histologic examination, Western blotting, immunohistochemical staining, and NIRF imaging using an MMP-activatable probe.
RESULTS
Multiple variable-sized tumors developed in both models and progressed from adenomas to adenocarcinomas over time. At the early stage of the AOM/DSS model, diffuse inflammation was observed within the tumors. MMP expression increased progressively through normal, inflammation, adenoma, and adenocarcionoma stages. NIRF signal intensities were strongly correlated with each tumor stage from adenoma to adenocarcinoma. NIRF imaging also distinguished tumors from inflamed mucosa.
CONCLUSIONS
NIRF imaging using a protease-activatable probe may be a useful tool for early tumor detection. This approach could translate to improve the endoscopic detection of colon tumors, especially in patients with inflammatory bowel disease.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Near-infrared Fluorescence Imaging Using a Protease-activatable Nanoprobe in Tumor Detection: Comparison with Narrow-band Imaging
- Near Infrared Autofluoroscence (NIRAF) in Thyroid Surgery
- Assessment and Comparison of Three Dimensional Exoscopes for Near-Infrared Fluorescence-Guided Surgery Using Second-Window Indocyanine-Green
- Near-Infrared Contrast Agents for Bone-Targeted Imaging
- Real-Time Localization of Parathyroid Glands with Near Infrared Light during Thyroid and Parathyroid Surgery
