Genomics Inform.  2016 Mar;14(1):12-19. 10.5808/GI.2016.14.1.12.

Neuropeptidomics: Mass Spectrometry-Based Identification and Quantitation of Neuropeptides

• Lee JE

• KMID: 2166535
• DOI: http://doi.org/10.5808/GI.2016.14.1.12

Abstract

Neuropeptides produced from prohormones by selective action of endopeptidases are vital signaling molecules, playing a critical role in a variety of physiological processes, such as addiction, depression, pain, and circadian rhythms. Neuropeptides bind to post-synaptic receptors and elicit cellular effects like classical neurotransmitters. While each neuropeptide could have its own biological function, mass spectrometry (MS) allows for the identification of the precise molecular forms of each peptide without a priori knowledge of the peptide identity and for the quantitation of neuropeptides in different conditions of the samples. MS-based neuropeptidomics approaches have been applied to various animal models and conditions to characterize and quantify novel neuropeptides, as well as known neuropeptides, advancing our understanding of nervous system function over the past decade. Here, we will present an overview of neuropeptides and MS-based neuropeptidomic strategies for the identification and quantitation of neuropeptides.

Keyword

identification; mass spectrometry; neuropeptides; peptidomics; quantitation

MeSH Terms

Circadian Rhythm
Depression
Endopeptidases
Mass Spectrometry
Models, Animal
Nervous System
Neuropeptides*
Neurotransmitter Agents
Physiological Processes
Vital Signs
Endopeptidases
Neuropeptides
Neurotransmitter Agents