Ann Lab Med.  2017 May;37(3):272-276. 10.3343/alm.2017.37.3.272.

Laboratory Identification of Leptotrichia Species Isolated From Bacteremia Patients at a Single Institution

Affiliations
  • 1Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. pmhhj77@gmail.com micro.lee@samsung.com
  • 2SD Genomics Co., Ltd., Seoul, Korea.

Abstract

We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level. Species level identification was successfully achieved by using 16S ribosomal RNA gene sequencing.

Keyword

Leptotrichia; Bacteremia; Identification; MALDI-TOF MS; 16S ribosomal RNA

MeSH Terms

Bacteremia*
Humans
Leptotrichia*
Mass Spectrometry
RNA, Ribosomal, 16S
RNA, Ribosomal, 16S
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