Exp Neurobiol.  2009 Jun;18(1):26-31. 10.5607/en.2009.18.1.26.

Effects of 6-hydroxydopamine on the Adult Neurogenesis of Dopaminergic Neurons in the Mouse Midbrain

• Kim TW
• Kim H
• Sun W

• KMID: 2165293
• DOI: http://doi.org/10.5607/en.2009.18.1.26

Abstract

Recently, restricted progenitor cells have been identified in the substantia nigra (SN) of the rat and mouse, raising a hope that resident stem/progenitor cells may be useful for the therapy of Parkinson's disease. However, it is controversial whether dopamine (DA) neurons can be spontaneously or injury-dependently generated from the endogenous stem cells in the adult brain. Here, we explored the neurogenesis in C57Bl/6 adult mice under the normal and neurotoxin-injured conditions. To monitor adult neurogenesis, we injected 5-bromodeoxyuridine (BrdU) 2 weeks after striatal injection of neurotoxin 6-hydroxydopamine (6-OHDA), and sacrificed the animals 6 weeks after 6-OHDA injection. Whereas the number of BrdU-labeled cells was slightly increased in ipsilateral side than contralateral side of the midbrain, none of BrdU- labeled cells, however, exhibited neuronal markers, NeuN or DCX. Instead, BrdU- labeled cells expressed glial markers such as GFAP (astrocyte), Olig2 (oligodendrocyte) and Iba-1 (microglia). Especially, larger portion of BrdU-labeled cells in the ipsilateral side exhibited microglial marker, indicating that increased cell production in response to the 6-OHDA injection is not related to the adult neurogenesis.

Keyword

Parkinson's disease; 6-OHDA; mice; adult neurogenesis; dopaminergic neurons

MeSH Terms

Adult
Animals
Brain
Bromodeoxyuridine
Dopamine
Dopaminergic Neurons
Humans
Mesencephalon
Mice
Neurogenesis
Neurons
Organothiophosphorus Compounds
Oxidopamine
Parkinson Disease
Rats
Stem Cells
Substantia Nigra
Bromodeoxyuridine
Dopamine
Organothiophosphorus Compounds
Oxidopamine