Int J Oral Biol.  2016 Mar;41(1):45-51. 10.11620/IJOB.2016.41.1.045.

Rutin induces autophagy in cancer cells

• Park MH
• Kim S
• Song YR
• Kim S
• Kim HJ
• Na HS
• Chung J

• KMID: 2162146
• DOI: http://doi.org/10.11620/IJOB.2016.41.1.045

Abstract

Rutin (3,3"²,4"²,5,7-pentahydroxyflavone-3-rhamnoglucoside) is a bioactive flavonoid from the plant kingdom. Rutin has been studied as potential anticancer agent due to its wide range of pharmacological properties including antioxidative, anti-inflammatory and anticancer. Autophagy is a conserved intracellular catabolic pathway to maintain cell homeostasis by formation of autophagosome. Processing of autophagy involves various molecules including ULK1 protein kinase complex, Beclin-1-Vps34 lipid kinase complex, ATG5, ATG12, and LC3 (light chain 3). Cargo-carried autophagosomes fuse with lysosomes resulting in autophagolysosome to eliminate vesicles and degrade cargo. However, the actions of rutin on autophagy are not clearly understood. In this study, we analyzed the effect of rutin on autophagy and inflammation in cancer cell lines. Interestingly, rutin induced autophagy in leukemia (THP-1), oral (CA9-22), and lung (A549) cell lines. TNF-α, key modulator of inflammation, was upregulated by inhibition of rutin-induced autophagy. Taken together, these data indicated that rutin induced autophagy and consequently suppressed TNF-α production.

Keyword

Rutin; autophagy; cancer cell

MeSH Terms

Autophagy*
Cell Line
Homeostasis
Inflammation
Leukemia
Lung
Lysosomes
Phosphotransferases
Plants
Protein Kinases
Rutin*
Phosphotransferases
Protein Kinases
Rutin