Maxillofac Plast Reconstr Surg.  2016 Mar;38(3):14. 10.1186/s40902-016-0060-6.

Chitin-fibroin-hydroxyapatite membrane for guided bone regeneration: micro-computed tomography evaluation in a rat model

• Baek YJ
• Kim JH
• Song JM
• Yoon SY
• Kim HS
• Shin SH

• KMID: 2161828
• DOI: http://doi.org/10.1186/s40902-016-0060-6

Abstract

BACKGROUND
In guided bone regeneration (GBR) technique, many materials have been used for improving biological effectiveness by adding on membranes. The new membrane which was constructed with chitin-fibroin-hydroxyapatite (CNF/HAP) was compared with a collagen membrane (Bio-Gide®) by means of micro-computed tomography.
METHODS
Fifty-four rats were used in this study. A critical-sized (8 mm) bony defect was created in the calvaria with a trephine bur. The CNF/HAP membrane was prepared by thermally induced phase separation. In the experimental group (n = 18), the CNF/HAP membrane was used to cover the bony defect, and in the control group (n = 18), a resorbable collagen membrane (Bio-Gide®) was used. In the negative control group (n = 18), no membrane was used. In each group, six animals were euthanized at 2, 4, and 8 weeks after surgery. The specimens were analyzed using micro-CT.
RESULTS
Bone volume (BV) and bone mineral density (BMD) of the new bone showed significant difference between the negative control group and membrane groups (P < 0.05). However, between two membranes, the difference was not significant.
CONCLUSIONS
The CNF/HAP membrane has significant effect on the new bone formation and has the potential to be applied for guided bone regeneration.

Keyword

Chitin-fibroin-hydroxyapatite; Guided bone regeneration; Micro-computed tomography; Rat calvarial defect

MeSH Terms

Animals
Bone Density
Bone Regeneration*
Collagen
Membranes*
Models, Animal*
Osteogenesis
Rats*
Skull
Collagen