J Vet Sci.  2015 Mar;16(1):25-29. 10.4142/jvs.2015.16.1.25.

Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems

• Shin MK
• Yoon SH
• Kim MH
• Lyoo YS
• Suh SW
• Yoo HS

• KMID: 2160817
• DOI: http://doi.org/10.4142/jvs.2015.16.1.25

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent for post-weaning, multisystemic, wasting syndrome. Consequently, serologic detection of and vaccination against PCV2 are important for the swine industry. Among several serological tests, the enzyme-linked immunosorbent assay (ELISA) is commonly used to measure anti-PCV2 antibody levels. In the present study, we used two commercial ELISA systems to comparatively evaluate anti-PCV2 antibodies in field pigs treated with three different PCV2 vaccines. Among a total of 517 serum samples, the results of the two ELISAs were fully concordant for 365 positive and 42 negative samples, indicating 78.7% agreement. In addition, the Pearson coefficient (0.636) indicated a moderate correlation between data from the two ELISAs. Results from the farms with pigs vaccinated with the three different PCV2 vaccines demonstrated that most of the vaccinated animals underwent seroconversion. However, the increase and duration of antibody titers varied depending on the vaccine, the presence of maternal antibodies, and the vaccination program. PCV2 serologic status and anti-PCV2 antibody levels of herds from this study could be utilized to determine the best timing for vaccination and assessing vaccination compliance.

Keyword

enzyme-linked immunosorbent assay; pigs; porcine circovirus 2; serology

MeSH Terms

Aging
Animals
Antibodies, Viral/*blood
Circovirus/*classification/immunology
Enzyme-Linked Immunosorbent Assay/methods/*veterinary
Female
Porcine Postweaning Multisystemic Wasting Syndrome/blood/immunology/*prevention & control
Republic of Korea/epidemiology
Swine
Swine Diseases/*prevention & control/virology
Viral Vaccines/*immunology
Antibodies, Viral
Viral Vaccines

Figure

• 

  Fig. 1 Comparison of data from the two available enzyme-linked immunosorbent assay (ELISA) kits using 517 field-collected pig sera. ELISA index values of the S-ELISA were square-root transformed. The regression equation is: y = -0.175x + 0.787; R-square = 0.405, p < 0.001.

• 

  Fig. 2 Detection of PCV2-specific antibodies in field pig serum samples from animals of different ages using two commercial ELISA kits. Anti-PCV2 antibodies were verified in samples from farms with pigs vaccinated with three different PCV2 vaccines (I, II, and III) using commercially available ELISA kits (S and M). The levels of PCV2-specific antibodies are shown in a box-and-whisker plot graph displaying the minimum, first quartile, median, third quartile, and maximum. The dotted lines indicate the median level for each farm. The y-axis represents the S/N ratio and S/P ratio for the S-ELISA and M-ELISA, respectively.

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