J Vet Sci.  2019 Jul;20(4):e35. 10.4142/jvs.2019.20.e35.

Enhanced protective immune response to PCV2 adenovirus vaccine by fusion expression of Cap protein with InvC in pigs

• Zhang Z
• Luo Y
• Zhang Y
• Guo K

• KMID: 2453897
• DOI: http://doi.org/10.4142/jvs.2019.20.e35

Abstract

The major immunogenic protein capsid (Cap) of porcine circovirus type 2 (PCV2) is critical to induce neutralizing antibodies and protective immune response against PCV2 infection. This study was conducted to investigate the immune response of recombinant adenovirus expressing PCV2b Cap and C-terminal domain of Yersinia pseudotuberculosis invasin (Cap-InvC) fusion protein in pigs. The recombinant adenovirus rAd-Cap-InvC, rAd-Cap and rAd were generated and used to immunize pigs. The phosphate-buffered saline was used as negative control. The specific antibodies levels in rAd-Cap-InvC and ZJ/C-strain vaccine groups were higher than that of rAd-Cap group (p < 0.05), and the neutralization antibody titer in rAd-Cap-InvC group was significantly higher than those of other groups during 21-42 days post-immunization (DPI). Moreover, lymphocyte proliferative level, interferon-γ and interleukin-13 levels in rAd-Cap-InvC group were increased compared to rAd-Cap group (p < 0.05). After virulent challenge, viruses were not detected from the blood samples in rAd-Cap-InvC and ZJ/C-strain vaccine groups after 49 DPI. And the respiratory symptom, rectal temperature, lung lesion and lymph node lesion were minimal and similar in the ZJ/C-strain and rAd-Cap-InVC groups. In conclusion, our results demonstrated that rAd-Cap-InvC was more efficiently to stimulate the production of antibody and protect pigs from PCV2 infection. We inferred that InvC is a good candidate gene for further development and application of PCV2 genetic engineering vaccine.

Keyword

Swine; circovirus; adenovirus vaccines; immunization; capsid protein

MeSH Terms

Adenoviridae*
Adenovirus Vaccines
Antibodies
Antibodies, Neutralizing
Capsid
Capsid Proteins
Circovirus
Genetic Engineering
Immunization
Interleukin-13
Lung
Lymph Nodes
Lymphocytes
Swine*
Yersinia pseudotuberculosis
Adenovirus Vaccines
Antibodies
Antibodies, Neutralizing
Capsid Proteins
Interleukin-13